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pET28a-mOct4 Full length(FL)

Plasmid

#206394

Purpose

Expresses Oct4 with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Insert

Oct4 (Pou5f1 Mouse)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Teto Ngn2

Plasmid

#62223

Purpose

Inducible expression of Ngn2

Depositor

Kristin Baldwin

Article

Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

Insert

Neurog2 (Neurog2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pR26-CMVconst-mBMP7

Plasmid

#127375

Purpose

Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

BMP7 (Bmp7 Mouse)

Use

CRISPR and Mouse Targeting

Promoter

CMV enhancer/promoter

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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mFGF8b

Plasmid

#22090

Depositor

Gail Martin

Article

Crossley et al Development. 1995 Feb . 121(2):439-51.

Insert

FGF8 (Fgf8 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pX335-EN477

Plasmid

#86232

Purpose

For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse CTCF STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Kv2.2

Plasmid

#231678

Purpose

Expression of mouse Kcnb2 fused with EGFP in C-terminal.

Depositor

Federico Sesti

Insert

Kcnb2 (Kcnb2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

None

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 raptor

Plasmid

#21339

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

raptor shRNA 1 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
Anti-HCN1 ion channel [N70/28R-1]

Plasmid

#206718

Purpose

Mammalian Expression Plasmid of anti-HCN1 ion channel (Rat). Derived from hybridoma N70/28-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN1 ion channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Hcn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-CA-CMV-GFP

Plasmid

#83255

Purpose

Adenoviral expression of constitutively active STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

constitutively active

Promoter

CMV

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

Plasmid

#203839

Purpose

Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mScarlet-KASH-2A

Mutation

Enzymatically inactive Cre

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3 basic Mut E2

Plasmid

#48748

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)

Use

Luciferase

Mutation

Mutated E-box2 sequence from CACGTT to GCTAGT

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-iRFP713-ORP9-PH

Plasmid

#214269

Purpose

Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

iRFP713-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pISH-Pax6

Plasmid

#74350

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pax6. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

paired box 6 (Pax6 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pP2BA

Plasmid

#31369

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Per2 (Per2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pSems StayGold-2xFYVE

Plasmid

#222948

Purpose

Expression of HRS 2xFYVE tagged with StayGold in mammalian cells

Depositor

Jacob Piehler

Article

Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

Plasmid

#184556

Purpose

Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

Depositor

Sandeep Prabhu

Article

Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

Insert

Kirsten rat sarcoma virus (Kras Mouse)

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tailless Control

Plasmid

#182718

Purpose

expresses a truncated force-insensitive DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al iScience. 2025 Jul 9;28(8):113081. doi:

Insert

desmoglein-3 (Dsg3 Mouse)

Expression

Mammalian

Mutation

TSmod inserted at G726, downstream C-terminal tai…

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV p2GM AMPK alpha2hp1 alpha1hp1

Plasmid

#89492

Purpose

shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

2 hairpins against mouse/human AMPKalpha1 and alpha2

Use

Retroviral

Expression

Mammalian

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pROSA26-5'

Plasmid

#21715

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

ROSA26-5' insert (Gt(ROSA)26Sor Mouse)

Use

For use with mouse targeting vector prosa26-1

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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