We narrowed to 8,864 results for: BLI;

1. Hs.KRAS4b K147L

   Plasmid

   #83154

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K147L mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   K147L

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Hs.KRAS4b Y64A

   Plasmid

   #83151

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y64A mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Y64A

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Hs.KRAS4b R68S

   Plasmid

   #83148

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R68S mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   R68S

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Hs.KRAS4b A146V

   Plasmid

   #83147

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146V mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   A146V

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Hs.KRAS4b K117N

   Plasmid

   #83146

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K117N mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   K117N

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Hs.KRAS4b M188L

   Plasmid

   #83141

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M188L mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   M188L

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK598

   Plasmid

   #72523

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK612

   Plasmid

   #72533

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK613

   Plasmid

   #72534

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK619

    Plasmid

    #72536

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 4QL1

    Plasmid

    #61942

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5:APC041-G05:C36415 (WDR5 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. 4MI0

    Plasmid

    #60097

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EZH2:JMC033-A03:C216172 (EZH2 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Expression

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pEL481

    Plasmid

    #52351

    Purpose

    GFP reporter for rack-1

    Depositor

    Erik Lundquist

    Article

    Demarco et al PLoS Genet. 2010 Nov 18;6(11):e1001215.

    Insert

    rack-1 (rack-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Flag YFP hNKCC1 V729C (NT503)

    Plasmid

    #50883

    Purpose

    Expresses human NKCC1 V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    V729C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Flag YFP hNKCC1 Y739C (NT525)

    Plasmid

    #50893

    Purpose

    Expresses human NKCC1 Y739C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    Y739C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 Flag YFP hNKCC1 C723S (NT537)

    Plasmid

    #50902

    Purpose

    Expresses human NKCC1 C723S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    C723S in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 Flag YFP hNKCC1 V740C (NT526)

    Plasmid

    #50894

    Purpose

    Expresses human NKCC1 V740C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    V740C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 Flag YFP hNKCC1 T738C (NT524)

    Plasmid

    #50892

    Purpose

    Expresses human NKCC1 T738C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    T738C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 Flag YFP hNKCC1 V729S (NT553)

    Plasmid

    #50911

    Purpose

    Expresses human NKCC1 V729S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    V729S in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 Flag YFP hNKCC1 W733C (NT507)

    Plasmid

    #50887

    Purpose

    Expresses human NKCC1 W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    W733C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1 Flag YFP hNKCC1 F728C (NT502)

    Plasmid

    #50882

    Purpose

    Expresses human NKCC1 F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    F728C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 Flag YFP hNKCC1 W732C (NT506)

    Plasmid

    #50886

    Purpose

    Expresses human NKCC1 W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    W732C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1 Flag YFP hNKCC1 L671C (NT440)

    Plasmid

    #50872

    Purpose

    Expresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L671C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)

    Plasmid

    #50871

    Purpose

    Expresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    E670C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MAP2K6

    Plasmid

    #38997

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAP2K6 (MAP2K6 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Activation loop mutant S207D/T211D

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

26. GLRX3

    Plasmid

    #39036

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GLRX3 (GLRX3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    A PCR artifact adds a C-terminal sequence includi…

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. DLG3

    Plasmid

    #38925

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. DLG3

    Plasmid

    #38929

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

29. human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

    Plasmid

    #39306

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E211A, K212A, E213A, R214A, D215A and Y217A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pBACgus N-His/GST/LRRK2 1867-2141

    Plasmid

    #40404

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Expression

    Mutation

    WT aa1867-2141

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pBACgus N-His/GST/LRRK2 1867-2161

    Plasmid

    #40405

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Expression

    Mutation

    WT aa1867-2161

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pBACgus4x/cdc37/LRRK2 1867-2161_C2024S/C2025S

    Plasmid

    #40372

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    HisTev

    Expression

    Mutation

    aa1867-2161, C2024S/C2025S

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S

    Plasmid

    #40388

    Depositor

    Michael J Fox Foundation MJFF

    Inserts

    SUMO
    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    6xHis and HisTEV

    Expression

    Mutation

    aa1867-2186, C2024S/C2025S

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. GMIP

    Plasmid

    #32046

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GMIP (GMIP Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 80-357 only

    Promoter

    Available since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

35. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198048

    Purpose

    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-CAG-GFP

    Plasmid

    #37825

    Depositor

    Edward Boyden

    Has Service

    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFP.R269

    Plasmid

    #138181

    Purpose

    Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FLAG-HA-pcDNA3.1-

    Plasmid

    #52535

    Purpose

    enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDB179-HotPETase

    Plasmid

    #218701

    Purpose

    Encodes codon optimized HotPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    HotPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198047

    Purpose

    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid

    #50474

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Mitochondrial PancACe

    Plasmid

    #215710

    Purpose

    Expresses acetyl-CoA biosensor in the mitochondria

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and mitochondrial localization sequence…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Nuclear PancACe

    Plasmid

    #215706

    Purpose

    Expresses acetyl-CoA biosensor in the nucleus

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit HC

    Plasmid

    #221355

    Purpose

    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit HC; to be used with Anti-Strep [IPI-C23.21] light chain (Plasmid 221356) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Strep [IPI-C23.21] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pc DNA FRT TO- APP695

    Plasmid

    #114193

    Purpose

    Creation of a stable cell line expressing APP mutation (AD in vitro study)

    Depositor

    Aleksandra Radenovic

    Insert

    Amyloid-Precursor-Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type splice variant 695: 695AA (2085bp, lack…

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Cytoplasmic PancACe

    Plasmid

    #215708

    Purpose

    Expresses acetyl-CoA biosensor in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. FLT3-ITD-MSCV-IRES-GFP

    Plasmid

    #184779

    Purpose

    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating internal tandem duplication (ITD) adde…

    Promoter

    MSCV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJMP1039

    Plasmid

    #119239

    Purpose

    expresses Tn7 transposase

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. AICSDP-079:DCP1A-mEGFP

    Plasmid

    #193922

    Purpose

    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

    Depositor

    Allen Institute for Cell Science

    Insert

    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid

    #221359

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge