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pQCXIP-Laforin Myc R171H

Plasmid

#84671

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pGEM-3z/611

Plasmid

#26661

Depositor

Jonathan Widom

Article

Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn1-synaptophysin-mCherry-V5

Plasmid

#250167

Purpose

Neuronal overexpression of synaptophysin fused to mCherry

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Synaptophysin

Expression

Mammalian

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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TV_ANXA1-p2a-mScarlet-nls-p2a-icasp9_pgk-blast

Plasmid

#251171

Purpose

Targeting vector for C-terminal knockin at ANXA1 locus to enable reporting of expression by mScarlet and depletion of positive cells with iCasp9.

Depositor

Hugo Snippert

Insert

p2a-mScarlet-nls-icasp9_pgk-blast flanked by homology arms for C-terminal targeting of human ANXA1 (ANXA1 Human)

Use

CRISPR

Tags

p2a-mScarlet-nls-p2a-icasp9

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

Plasmid

#251173

Purpose

Targeting vector for C-terminal knockin at LGR5 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR

Depositor

Hugo Snippert

Insert

ires-mNeon-nls-p2a-dtr-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human LGR5 (LGR5 Human)

Use

CRISPR

Tags

ires-mNeon-nls-p2a-dtr

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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EML8552_pEML41003-tufApst4-15

Plasmid

#237865

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufA

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufA

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8553_ pEML41003-tufBpst4-15

Plasmid

#237866

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufB

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufB

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(WT, Y371C)-HA

Plasmid

#239765

Purpose

Expresses TTX-resistant (Y371C) variant of mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

Y371C

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-FL-N-FLAG

Plasmid

#222675

Purpose

overexpressing full length NRBP1 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-TSC22D2-Nterm-C-FLAG

Plasmid

#222683

Purpose

overexpressing N-terminus truncation of TSC22D2 with C-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

TSC22D2 N-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

Plasmid

#225435

Purpose

BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

LIR1:35S:eGFP:T35S-SIR:LIR2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

Plasmid

#217799

Purpose

Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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p2701-AGER-tdTomato

Plasmid

#216470

Purpose

donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

tdTomato

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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53BP1 KO sgRNA

Plasmid

#207104

Purpose

pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGATTCTCAGCCTGAAAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAC09

Plasmid

#197403

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and RSF1010 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

RESF1010 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pIS001

Plasmid

#194012

Purpose

This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

Depositor

Timothy Whitehead

Article

Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

Insert

ROSA-TRE-C16V5-G-pA

Expression

Bacterial

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLX208-nfBFP

Plasmid

#171013

Purpose

Ectopic expression of nonfluorescent BFP

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

untagged nfBFP

Use

Lentiviral

Expression

Mammalian

Mutation

BFP[H66A]

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBW1345_pCAG-PV1-PhiC31-N396-L1-FRB-NLS-BGHpA

Plasmid

#108791

Purpose

Expresses N-terminus to aa396 of PhiC31 integrase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment N-terminus to aa396

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1358_pCAG-PV1-FKBP-L1-PhiC31-397C-NLS-BGHpA

Plasmid

#108792

Purpose

Expresses aa397 to C-terminus of PhiC31 integrase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpA

Plasmid

#108749

Purpose

Expresses N-terminus to aa168 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa168

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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