We narrowed to 9,406 results for: Coli

1. B0032m_BC

   Plasmid

   #66020

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ02B2Gm_EF

   Plasmid

   #66064

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6_Ptrc_BsMdh_BmHps_BmPhi

   Plasmid

   #129104

   Purpose

   Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

   Depositor

   Eleftherios Terry Papoutsakis

   Article

   Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

   Insert

   BsMDH BmHPS BmPHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pFA-sfGFP

   Plasmid

   #162689

   Purpose

   pFA vector control expressing sfGFP

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   sfGFP
   

   Use

   Tags

   C terminal 6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   PL tet promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SLIRP.HMG6.wt.fl

   Plasmid

   #49821

   Purpose

   expression vector for MBP-SLIRP fusion

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
   

   Use

   Tags

   E. coli Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAL950

   Plasmid

   #119748

   Purpose

   precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Internal NdeI site removed and mutations Alanine …

   Promoter

   T7

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTTR210K

   Plasmid

   #41750

   Depositor

   Jeff Gelles

   Article

   Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

   Insert

   kinesin heavy chain (Khc Fly, E. coli)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; R210K

   Promoter

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)

   Plasmid

   #70194

   Purpose

   Derived from 21 with silent mutations to make BsaI, BbsI compatible

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6his R.3-AR GUSpET28a+/pLysS

   Plasmid

   #61157

   Purpose

   N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

   Depositor

   Andrew Ellington, Ichiro Matsumura

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   GUSAR
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

   Promoter

   T7

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE76

    Plasmid

    #80578

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLaNGo11-3

    Plasmid

    #126054

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMiNGo11-1

    Plasmid

    #126056

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-Pc4CL-PhCHS-CmCHI

    Plasmid

    #73397

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEM-Cas9noSsrA

    Plasmid

    #102285

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    removed ssrA tag

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE77

    Plasmid

    #80579

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE78

    Plasmid

    #80580

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE79

    Plasmid

    #80581

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFP121

    Plasmid

    #21670

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pBN415

    Plasmid

    #217920

    Purpose

    express E. coli E69 (O9a:K30) Wzc with N711Y mutation

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with N711Y mutation
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    N711Y

    Promoter

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYihI-C-term K-R

    Plasmid

    #233092

    Purpose

    Expression of GST-YihI with C-term lysines (K) mutated to arginine (R)

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    GST-YihI with C-term lysines (K) mutated to arginine (R)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C-term lysines (K) mutated to arginine (R)

    Promoter

    Available Since

    May 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pVF18

    Plasmid

    #229456

    Purpose

    Expresses IraD (aa17-130) with His-tag

    Depositor

    Alexandra Deaconescu

    Article

    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

    Insert

    IraD (aa 17-130 only)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 17-130 only

    Promoter

    T7

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. SBC015898

    Plasmid

    #226285

    Purpose

    Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

    Depositor

    Rainer Breitling

    Article

    Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    Eugenol O-methyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRetroX-TRE3G-Tir

    Plasmid

    #221326

    Purpose

    Dox-inducible expression of Tir in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    Tir
    

    Use

    Retroviral

    Tags

    HN-FLAG

    Expression

    Mutation

    Promoter

    TRE3GV

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pWR88

    Plasmid

    #203422

    Purpose

    empty BLINCAR plasmid for payload delivery, no payload, no genomic target

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    CAR copy 1
    P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
    CAR copy 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMBA337

    Plasmid

    #214750

    Purpose

    Medium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validation

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE-PGUS

    Plasmid

    #207827

    Purpose

    FLAG-tagged PGUS driven by CMV promoter

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    PGUS
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid

    #201534

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE_RBS3_CB3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    rhaS W190G

    Promoter

    PrhaBAD (rhamnose)

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pL0_CD-GUS

    Plasmid

    #202137

    Purpose

    Level 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Beta glucuronidase gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pYG215

    Plasmid

    #200837

    Purpose

    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.

    Depositor

    Boris Görke

    Article

    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

    Insert

    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    P-LlacO-1

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTP-PCR C1

    Plasmid

    #189618

    Purpose

    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Promoter

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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