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Showing: 5601 - 5640 of 9865 results

  4. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid
    #140977
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoB-Rluc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140977-plasmid-map-sequence-id-300660

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  6. pFP.E222

    Plasmid
    #138248
    Purpose
    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138248-plasmid-map-sequence-id-273451

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  9. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

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  15. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid
    #73206
    Purpose
    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-TM (MYC Human)
    Tags
    The FAP is fused with 2 copies of a G4S linker an…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73206-plasmid-map-sequence-id-139721

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  21. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid
    #73205
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    NLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73205-plasmid-map-sequence-id-139718

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  36. pCB268

    Plasmid
    #53363
    Purpose
    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.
    Depositor
    Alexander Varshavsky
    Article
    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:
    Inserts
    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    Tags
    13xMyc, Flag, and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5601 - 5640 of 9865 results