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pDmelOR-mRho.V5.mER.hOr47a

Plasmid

#126478

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or47a (Or47a Fly)
Orco (Orco Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry

Plasmid

#180646

Purpose

Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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acta2_mCherry_pA_pDestTolpA2

Plasmid

#78685

Purpose

acta2:mCherry transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

mCherry

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-2 pMT2

Plasmid

#58732

Purpose

expression of mouse Alpha2 delta-2 in mammalian cells

Depositor

Annette Dolphin

Article

Barclay et al J Neurosci. 2001 Aug 15;21(16):6095-104.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pBSSK p85 alpha (delta BH)

Plasmid

#13431

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta BH) (PIK3R1 Human)

Tags

FLAG

Expression

Bacterial

Mutation

BH domain replaced by flag tag.

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAV-U6+27-Tornado-RhoBAST

Plasmid

#196341

Purpose

Overexpression of circular RhoBAST

Depositor

Murat Sunbul

Article

Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

Insert

Tornado-RhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV7 mitoDendra2

Plasmid

#157757

Purpose

Expression of the fluorescent protein Dendra2 targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

Dendra2

Expression

Mammalian

Mutation

MTS coxVIII +Dendra2

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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dCBE4-gam

Plasmid

#124449

Purpose

Expresses dead Cas9 (dCas9)-CBE4-gam

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dCBE4-gam

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Expression

Bacterial and Yeast

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CAMKK1

Plasmid

#20442

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CAMKK1 (CAMKK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-ALK6 Q203D

Plasmid

#80883

Purpose

mammalian expression of ALK6 Q203D

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Mutation

Q203D (constitutively active)

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
mNeonGreen-3xrGBD

Plasmid

#176091

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC9A3

Plasmid

#132234

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A3 (SLC9A3 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A3

Plasmid

#132040

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A3 (SLC25A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A5

Plasmid

#132007

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A5 (SLC25A5 Human)

Expression

Mammalian

Available Since

Oct. 24, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-ULK3

Plasmid

#23646

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK3 (ULK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pLEX307 hDVL1

Plasmid

#102863

Purpose

Expresses human DVL1 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

human Dishevelled 1 (DVL1) (DVL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 2 to glycine. Likely due to poly…

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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