166,034 results

1. EF1a mCherry

   Plasmid

   #129431

   Purpose

   expression of mCherry from EF1a promoter

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pH-ePPE

   Plasmid

   #183097

   Purpose

   For plant prime editing in rice plants

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-F-del Casp9.IRES.GFP

   Plasmid

   #15567

   Depositor

   David Spencer

   Article

   Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

   Insert

   FKBP12(V36)-p30Caspase9 (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   FKBP12 and HA epitope

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

   Available Since

   Aug. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

   Plasmid

   #140003

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Ubc.Luc.IRES.Puro

   Plasmid

   #33307

   Depositor

   Linzhao Cheng

   Article

   Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lamp1_mStayGold

   Plasmid

   #221902

   Purpose

   Expresses mStayGold fused to Lamp1

   Depositor

   Janelia Integrative Imaging

   Insert

   Lamp1_mStayGold
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE2-TetOminiCMV-SV40LT

   Plasmid

   #136616

   Purpose

   Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   SV40 large T antigen
   

   Use

   Lentiviral

   Expression

   Bacterial

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

   Plasmid

   #134867

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ABE8.20-SpRY

   Plasmid

   #226859

   Purpose

   Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8.20-SpRY
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRL-SV40P

    Plasmid

    #27163

    Purpose

    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

    Depositor

    Ron Prywes

    Article

    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

    Insert

    SV40 promoter
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBtet-GH

    Plasmid

    #60498

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Available Since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHEN2-SG4

    Plasmid

    #196071

    Purpose

    Express wild type SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4
    

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. v5 ABE-eVLP

    Plasmid

    #228467

    Purpose

    Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–ABE8e
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1-AR

    Plasmid

    #28235

    Purpose

    Mammalian expression of Androgen receptor fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3- SREBP-1

    Plasmid

    #204192

    Purpose

    Mammalian expression of SREBP-1 (isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Tags

    E-tag and T7x2

    Expression

    Mammalian

    Mutation

    multiple silent mutations

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge