We narrowed to 7,518 results for: Lif

1. pcDNA5/FRT/TO_b4g-HaloTag9

   Plasmid

   #175546

   Purpose

   Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Beta4Gal-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1_3XFlag

   Plasmid

   #182494

   Purpose

   Empty plasmid containing Flag tag for C-terminal tagging

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

   Insert

   3xFlag with 5 Glycolic spacer
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

   Plasmid

   #175530

   Purpose

   Expression of HaloTag7 in the endoplasmid reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag7-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET246-pUC57 12xMS2V6

   Plasmid

   #104390

   Purpose

   MS2V6 cloning

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

   Plasmid

   #175544

   Purpose

   Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag9-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRSV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EFS-MCP-HALOnls

   Plasmid

   #121937

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   MCP-HALOnls
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Use

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-mTagBFP2-T2A-iCre-ERT2

    Plasmid

    #128168

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPUR-hU6-sgRNA-Sirius-8XMS2

    Plasmid

    #121942

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57 mini-ZsGreen

    Plasmid

    #222648

    Purpose

    Vector for cloning gene of interest for in vitro transcription

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    ZsGreen
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    mutated stop codon

    Promoter

    T7

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-TRV1

    Plasmid

    #180515

    Purpose

    Agrobacterium tumefaciens-based expression of TRV1

    Depositor

    Jose-Antonio Daros

    Article

    Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

    Insert

    Tobacco rattle virus RNA1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S CaMV

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_LAMP1_HaloTag9

    Plasmid

    #175541

    Purpose

    Lyzosomal expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSAD-F3-NPEST-iCRE-2A-mCherryPEST

    Plasmid

    #99608

    Purpose

    Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    iCRE
    mCherry
    

    Use

    Unspecified

    Tags

    PEST

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid

    #225250

    Purpose

    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

    Plasmid

    #175536

    Purpose

    Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-SUPREM

    Plasmid

    #205023

    Purpose

    Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    SUPer Rna Ecogii Methyltransferase
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX-EF1a_NKX2-1_Long

    Plasmid

    #221483

    Purpose

    NKX2-1 long isoform lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

    Plasmid

    #175540

    Purpose

    Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag9-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CAG-Peredox

    Plasmid

    #73807

    Purpose

    Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

    Depositor

    Gary Yellen

    Article

    Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

    Insert

    Peredox
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(-)-qmTQ2-ATP

    Plasmid

    #225248

    Purpose

    mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDH-TMEM106b-mApple

    Plasmid

    #179385

    Purpose

    Lentiviral vector for expression of TMEM106b fused mApple

    Depositor

    Angus Johnston

    Article

    Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

    Insert

    TMEM106b - mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

    Plasmid

    #175539

    Purpose

    Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5/FRT/TO_HaloTag9_LamB1

    Plasmid

    #175538

    Purpose

    Expression of HaloTag9 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCyRFP1-Cdc42

    Plasmid

    #84360

    Purpose

    fusion protein of Cdc42, FRET donor

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1-Cdc42
    

    Use

    Tags

    mCyRFP1 N terminal fusion to Cdc42

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD/HisB (TIREVOL)-mNG

    Plasmid

    #189722

    Purpose

    titratable expression of mNeonGreen in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mNeonGreen
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRsetB-RoTq-Off

    Plasmid

    #234466

    Purpose

    Bacterial expression vector for RoTq-Off

    Depositor

    Gary Yellen

    Article

    Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

    Insert

    RoTq-Off
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-EFS-PCP-GFPnls

    Plasmid

    #121938

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    PCP-GFPnls
    

    Use

    Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge