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Showing: 7051 - 7100 of 9723 results

  5. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Tags
    6xHis
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available Since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

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  9. pSEVA3b61

    Plasmid
    #58310
    Purpose
    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    58310-plasmid-map-sequence-id-89822

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  31. wt Cus2p

    Plasmid
    #73046
    Purpose
    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Promoter
    T7
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    73046-plasmid-map-sequence-id-150790

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  50. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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Showing: 7051 - 7100 of 9723 results