We narrowed to 32,062 results for: eng

1. pYPQ265H

   Plasmid

   #149554

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   hAID-ziSpyMacCas9(D10A)-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ266B

   Plasmid

   #149555

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   ziSpyMacCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ290-D570A-SRDX

   Plasmid

   #129682

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b-SRDX
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Expression

   Plant

   Mutation

   D570A; AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ291-D573A-SRDX

   Plasmid

   #129683

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b-SRDX
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Expression

   Plant

   Mutation

   D573A; BthCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBTK110

   Plasmid

   #110576

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP6 Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pBTK112

   Plasmid

   #110577

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP12b Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBTK113

   Plasmid

   #110578

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP32 Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. p3E-U6a-U6c-sod2 guide

   Plasmid

   #107604

   Purpose

   An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   sod2 guide RNA sequences
   

   Use

   CRISPR

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-U6a-U6c-sod1 guide

   Plasmid

   #107603

   Purpose

   An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   sod1 guide RNA sequences
   

   Use

   CRISPR

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-U6a-U6c-uqcrc1 guide

    Plasmid

    #107602

    Purpose

    An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    uqcrc1 guide RNA sequences
    

    Use

    CRISPR

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMV-TRP1

    Plasmid

    #65335

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TRP1
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gal4pBS3-Pleum

    Plasmid

    #60330

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Gal4pBS1-Pleum

    Plasmid

    #59993

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMXs-B5

    Plasmid

    #31709

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-363 (Mir363 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pNTPTP alpha (S204A)

    Plasmid

    #17699

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S204A (PTPRA Human)
    

    Expression

    Mammalian

    Mutation

    S204A

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-hsyn-GRAB_DA2h

    Plasmid

    #140554

    Purpose

    Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.GFAP.(Cyto)iGlucoSnFR

    Plasmid

    #164513

    Purpose

    Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

    Depositor

    Loren Looger

    Article

    Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

    Insert

    (Cyto)iGlucoSnFR
    

    Use

    AAV

    Mutation

    Synthetic Construct

    Promoter

    GFAP

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid

    #122650

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    PylRS-AF, Pyl-tRNACUA
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-fDIO-GFP-IRES-tTA

    Plasmid

    #118026

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-fDIO-GFP-IRES-tTA
    

    Use

    AAV

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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