We narrowed to 14,031 results for: TIM

1. Jonah-1

   Plasmid

   #228899

   Purpose

   Jonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFP

   Depositor

   John Samuelson

   Article

   Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

   Insert

   Jonah-1
   

   Use

   Acanthamoeba expression vector

   Tags

   eGFP

   Mutation

   none

   Promoter

   Own promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Leo-A

   Plasmid

   #228901

   Purpose

   Leo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFP

   Depositor

   John Samuelson

   Article

   Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

   Insert

   Leo-A
   

   Use

   Acanthamoeba expression vector

   Tags

   eGFP

   Mutation

   none

   Promoter

   Own

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFLEX+mCyclin D1

   Plasmid

   #86793

   Purpose

   mammalian expression of cyclin D1

   Depositor

   Martine Roussel, Charles Sherr

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D1 (Ccnd1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-LacI-NLS-VP16

   Plasmid

   #103836

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   EGFP-LacI-NLS-VP16
   

   Expression

   Mammalian

   Promoter

   SV2

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB2-DsRed-Express2

   Plasmid

   #103999

   Purpose

   Plasmid encoding DsRed-Express2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   DsRed-Express2
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

   Plasmid

   #20946

   Purpose

   3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   halorhodopsin
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2,0_3E5E_eGFP

   Plasmid

   #69359

   Purpose

   Encodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

   Insert

   3E5E eGFP minigenome
   

   Use

   T7

   Promoter

   T7

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYZ033

   Plasmid

   #98404

   Purpose

   Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pESC-URA::CYP71AV1/CPR

    Plasmid

    #20118

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Insert

    amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 2, 2009

    Availability

    Academic Institutions and Nonprofits only

11. tetO-Myocd

    Plasmid

    #46033

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    Myocd (Myocd Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-FLEX-CAG-CyRFP1

    Plasmid

    #84357

    Purpose

    Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Double FLEXed

    Promoter

    CAG

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPaxillin-Electra2

    Plasmid

    #184926

    Purpose

    Expresses paxillin fused to blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    paxillin-Electra2
    

    Tags

    Electra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKeratin-Electra1

    Plasmid

    #179480

    Purpose

    Keratin labeling with blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Keratin-Electra1
    

    Tags

    Electra1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_NES_STf

    Plasmid

    #182001

    Purpose

    CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    NES-STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PE_CO

    Plasmid

    #190335

    Purpose

    Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

    Depositor

    Rasmus Bak

    Article

    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

    Insert

    PE2 with codon-optimized reverse transcriptase
    

    Expression

    Mammalian

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LAMP-CeNL_pcDNA3

    Plasmid

    #89514

    Purpose

    Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Tags

    LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cry2(1-531)-mCh-BAXS184E

    Plasmid

    #117238

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531-mCh-BAXS184E
    

    Expression

    Mammalian

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKB083

    Plasmid

    #181952

    Purpose

    IPTG-inducible expression of PhoQ

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
    

    Expression

    Bacterial

    Promoter

    PhoQ-Ptac; lacI-PlacIq

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Promoter

    35SPPDK promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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