12,254 results

1. pOpen-Eco31IB

   Plasmid

   #165527

   Purpose

   Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Eco31IB
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

   Plasmid

   #191529

   Purpose

   Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   H2BK_mNG_hSLBP(18-108)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mNeonGreen (mNG)

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2+E-Luc

   Plasmid

   #170873

   Purpose

   Phytobrick - E-Luc Luciferase CDS

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Mutation

   c.375C>T to remove BbsI site

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLtR5

   Plasmid

   #167505

   Purpose

   Broad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   TetR-RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLtetO-1

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pf CAG mc2 IRES blast

   Plasmid

   #206235

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA_Level 1A

   Plasmid

   #102701

   Purpose

   Mobius Assembly Level 1 Acceptor Vector A

   Depositor

   Naomi Nakayama

   Article

   Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

   Insert

   J23106:B0034:spisPink:rrnBT1-T7TE
   

   Use

   Synthetic Biology

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-P4

   Plasmid

   #120866

   Purpose

   Encodes permuteposon P4 for MuA transposase-mediated circular permutation

   Depositor

   Jonathan Silberg

   Article

   Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

   Insert

   Permuteposon P4
   

   Use

   Synthetic Biology

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRF-LmrA

   Plasmid

   #49364

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   LmrA repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AP-PTEN(med)

   Plasmid

   #168005

   Purpose

   positive selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   PTEN-T7PAP
   

   Use

   Synthetic Biology

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS154

    Plasmid

    #194477

    Purpose

    synAR Part (Part 3)

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    synAR (Part 3)
    

    Use

    Synthetic Biology

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS167

    Plasmid

    #194490

    Purpose

    37-12 Op (Part 2)

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    37-12 Op (Part 2)
    

    Use

    Synthetic Biology

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS166

    Plasmid

    #194489

    Purpose

    43-8 Op (Part 2)

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    43-8 Op (Part 2)
    

    Use

    Synthetic Biology

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB2H2*

    Plasmid

    #163834

    Purpose

    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    TCPTP R222M

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pf CAG mc2 IRES puro

    Plasmid

    #206233

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOSV807

    Plasmid

    #126600

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-HSlig3

    Plasmid

    #165574

    Purpose

    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    DNA Ligase III
    

    Use

    Synthetic Biology

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pgRNAtet-phaZ

    Plasmid

    #169874

    Purpose

    Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards phaZ in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPTK006-3a-αMF no EAEA

    Plasmid

    #84965

    Purpose

    Type 3a, α-mating factor no EAEA - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor no EAEA secretion tag
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Non

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. P16ΔS:GFP1–9

    Plasmid

    #108186

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components (GFP1-9) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Insert

    GFP1-9
    

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    P16ΔS

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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