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Myc-gamma1/gamma2SIL

Plasmid

#121432

Purpose

GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Insert

GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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rat brain inositol 1,4,5-trisphosphate 3-kinase A

Plasmid

#122155

Purpose

rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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gh151

Plasmid

#106821

Purpose

expression of gRNA targeting GLT8D1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GLT8D1 (GLT8D1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh98

Plasmid

#106766

Purpose

expression of gRNA targeting LFNG

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LFNG (LFNG Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-KD

Plasmid

#114066

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-KD (TTK Human)

Tags

GST

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-WT

Plasmid

#114065

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-WT (TTK Human)

Tags

GST

Expression

Insect

Mutation

none

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPN112

Plasmid

#91639

Purpose

Express sgRNA targeting human MAN2A1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MAN2A1 (MAN2A1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN113

Plasmid

#91640

Purpose

Express sgRNA targeting human MAN2A1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

MAN2A1 (MAN2A1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSN34

Plasmid

#100119

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to 2 VP16 domains (413-460) separated by linkers (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSN21

Plasmid

#100106

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSN61

Plasmid

#100122

Purpose

expresses putative OCT4 DBD with a T7 tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-putative OCT4 DBD (138-289) with a T7 tag (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSN35

Plasmid

#100120

Purpose

expresses a chimeric OCT4-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSN31

Plasmid

#100116

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSN32

Plasmid

#100117

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del3

Plasmid

#96913

Purpose

Murine Atoh1 with amino acids 214-305 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 214-305

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del4

Plasmid

#96914

Purpose

Murine Atoh1 with amino acids 306-347 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 306-347

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-chCAR

Plasmid

#87231

Purpose

Mammalian expression of chicken CAR

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Mc2r-mod

Plasmid

#48173

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Mutation

A/G MUTATION IN ATG OF UORF

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Kdr-mod

Plasmid

#48177

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of 2KDR (MC2R Human)

Use

Luciferase

Mutation

A/G MUTATION IN ATG OF UORF

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
5mARF17 pBluescript

Plasmid

#12069

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

5mARF17 (ARF17 Mustard Weed)

Expression

Bacterial

Mutation

miR160-resistant version of ARF17 has five silent…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-h53BP1 (siRNA resistant)

Plasmid

#110301

Purpose

Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

GAACGAGGA to GAGCGGGGC

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCbs FlagOmomyc

Plasmid

#113168

Purpose

Expression of FLAG tagged Omomyc in mammalian cells

Depositor

Sergio Nasi

Article

Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

Insert

Omomyc (MYC Human)

Tags

FLAG

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta2

Plasmid

#166772

Purpose

Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GNB2 (GNB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta4

Plasmid

#166773

Purpose

Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GNB4 (GNB4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 WT

Plasmid

#186472

Purpose

Expression of human MOSPD2 fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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