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Plasmid#74459PurposeMonitor post-translational degradation of Aiolos AA130-189 via EGFP:mCherry ratio (Lentiviral SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry)DepositorInsertAiolos (IKZF3) AA130-189 (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1515
Plasmid#62385PurposepFA6a Osm1-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationPromoterAdh1pAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGA4-GFP
Plasmid#81178PurposeMammalian expression of human full length ITGA4-EGFPDepositorInsertITGA4 (ITGA4 Human)
UseTagsEGFPExpressionMammalianMutationContains KLGFFKR motif; A1002L (please see deposi…PromoterCMVAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJA291
Plasmid#74487PurposeeF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFPDepositorInsertpuroR-mCherry-T2A-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1517
Plasmid#62387PurposepFA6a Osm1(M33A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationMethionine 33 mutated to AlaninePromoterAdh1pAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1516
Plasmid#62386PurposepFA6a Osm1(M1A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
UseTagsEGFPExpressionYeastMutationMethionine 1 to AlaninePromoterAdh1pAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
UseTagsFlag + eGFPExpressionBacterialMutationPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL2108
Plasmid#201969PurposeORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.DepositorInsertattB-3C-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
7TG
Plasmid#24314DepositorInsert7xTcf-eGFP
UseLentiviralTagsExpressionMutationEGFP is GFP with S65T and F64L mutationsPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNSEN-d2
Plasmid#59763PurposeExpresses nuclear EGFP and Tomato in mammalian cellsDepositorInsertsd2-EGFP
Tomato
UseTags3X NLS and PESTExpressionMammalianMutationPromoterCMV promoter and SV40 promoterAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT39
Plasmid#204289PurposeFor marking CRISPR HDR integrant with EGFP expressed in flight muscles.DepositorInsertMHC-EGFP+H3:H26
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDT305
Plasmid#128801PurposeHuman codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backboneDepositorInsertdCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
UseCRISPR and LentiviralTagsExpressionMutationPromoterhUBC promoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT-Pan
Plasmid#99638PurposeEGFP-mCherry control reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAG-Pan
Plasmid#99640PurposeEGFP-mCherry Met71GAG reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationMet71 mutated to GAG (Glu)PromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAA-Pan
Plasmid#99639PurposeEGFP-mCherry Met71GAA reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationMet71 mutated to GAA (Glu)PromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-05
Plasmid#119984PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT75
Plasmid#204306PurposeFlippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthpartsDepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-N2cG
Plasmid#192838Purposeplasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorInsertmTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
UseAAVTagsExpressionMammalianMutationPromoterTREtightAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPS-hSox2-gfp
Plasmid#49390Purposemammalian expression of human Sox2 fused to EGFPDepositorInsertSox2 (SOX2 Human)
UseCre/Lox; Acceptor vectorTagsEGFPExpressionMammalianMutationTGA (stop) to CCA (Pro) at nt 1027 with respect t…PromoterCMVAvailable sinceNov. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJAT43
Plasmid#204299PurposeFor introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03
Plasmid#67137PurposeGateway destination vector for cell-free expression with N-term 8xHis eGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-ACTLg
Plasmid#61195PurposeFluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoterDepositorInsertLifeAct-eGFP
UseTagseGFPExpressionPlantMutationPromoterMtBCP1Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G04
Plasmid#67138PurposeGateway destination vector for cell-free expression with C-term eGFP 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HISExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-Lifeact-GFP
Plasmid#175437PurposeF-actin structures labellingDepositorInsertLifeact-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterpTub-alpha1Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX1418
Plasmid#127856PurposeExpresses EGFP-IPNN-MCAK in mammalian cellsDepositorInsertIPNN-MCAK
UseTagsEGFPExpressionMammalianMutationmutation is I97N and P98NPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-eab2-GG
Plasmid#182154PurposeExpression of artificial intron separated eGFPDepositorInsertGG
UseZebrafish expressionTagsExpressionMutationPromotereab2Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX1433
Plasmid#127857PurposeExpresses EGFP-deltaN-term-MCAK in mammalian cellsDepositorInsertdeltaN-term-MCAK
UseTagsEGFPExpressionMammalianMutationdeletion of first 19 codons of MCAKPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPS-hAkt1-gfp
Plasmid#49388Purposemammalian expression of human Akt1 fused to EGFPDepositorInsertAkt1 (AKT1 Human)
UseCre/Lox; Acceptor vectorTagsEGFPExpressionMammalianMutationtga to cca at nt 1725 with respect to GenBank ID …PromoterCMVAvailable sinceNov. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAWV
Plasmid#62710PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInserttdTomato/amiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZA45
Plasmid#158523PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWK
Plasmid#62713PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInsertamiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
vsv-Sgo1-GFP
Plasmid#108494Purposeexpression of vsv-Sgo1-EGFPDepositorInsertShugoshin 1 (SGO1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-PE
Plasmid#92126PurposeExpression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reeseiDepositorInsertCas9-eGFP
UseCRISPR; Trichoderma reesei expressionTagsEGFPExpressionMutationPromoterPpdcAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorInsertEGFP-PEST-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-CE
Plasmid#92128PurposeExpression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reeseiDepositorInsertCas9-eGFP
UseCRISPR; Trichoderma reesei expressionTagsEGFPExpressionMutationPromoterPcbh2Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDAS12395_PEAR-GFP_2.0-preedited
Plasmid#177185PurposePEAR-GFP3 control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV2_HLF-ZE
Plasmid#175034PurposeHLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codonDepositorInsertHLF ires ZsGreen P2A tEGFR
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z05
Plasmid#120048PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAS12125_PEAR-GFP
Plasmid#177178PurposePEAR-GFP target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fluorescent reporter for MOBE1-2
Plasmid#219945PurposeMammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2DepositorInsertCMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
UseCRISPRTagsExpressionMammalianMutationEGFP(A111V/L202S)PromoterCMV, U6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDAS12124_PEAR-GFP-preedited
Plasmid#177179PurposePEAR-GFP control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)1
Plasmid#127089PurposeBacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP human SUMO3 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fluorescent reporter for MOBE3-4
Plasmid#219946PurposeMammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4DepositorInsertCMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
UseCRISPRTagsExpressionMammalianMutationEGFP(A111V/L202S)PromoterCMV, U6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMP4657
Plasmid#107773PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pME6010
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PECAM-H2B-GFP
Plasmid#14689DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsH2B-GFPExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ macroH2A1.2-GFP-HA
Plasmid#30515DepositorInsertmacroH2A1.2-C (Macroh2a1 Mouse)
UseXenopus/zebrafish expression.TagseGFP and triple HAExpressionMammalianMutationNO STOPPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only