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We narrowed to 12,157 results for: pcDNA

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Showing: 7201 - 7250 of 12157 results

  2. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

    Plasmid
    #47387
    Purpose
    allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin
    Depositor
    Henry Lester
    Article
    Frazier et al J Biol Chem. 2013 May 29.
    Insert
    glutamate-gated chloride Channels
    Expression
    Mammalian
    Mutation
    Leu9 prime mutated to F (Phenylalanine in M3-M4 l…
    Promoter
    CMV
    Available Since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    47387-plasmid-map-sequence-id-67454

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  4. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid
    #206121
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV
    Available Since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206121-plasmid-map-sequence-id-410287

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  7. pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

    Plasmid
    #50896
    Purpose
    Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L717C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50896-plasmid-map-sequence-id-76369

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  13. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

    Plasmid
    #166814
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E1322R
    Promoter
    CMV
    Available Since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166814-plasmid-map-sequence-id-331962

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  14. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid
    #166811
    Purpose
    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    LL1193EE, P1214R, K1277E
    Promoter
    CMV
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166811-plasmid-map-sequence-id-328312

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  15. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid
    #166812
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    H1276G
    Promoter
    CMV
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166812-plasmid-map-sequence-id-328391

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  16. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

    Plasmid
    #166813
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K1277E
    Promoter
    CMV
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166813-plasmid-map-sequence-id-328384

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  18. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid
    #155313
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155313-plasmid-map-sequence-id-310080

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  19. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid
    #155009
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155009-plasmid-map-sequence-id-309962

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  23. pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

    Plasmid
    #83458
    Purpose
    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)
    Depositor
    Thomas Weimbs
    Article
    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do
    Insert
    Polycystin-1 (PKD1 Human)
    Tags
    6xHis and Myc
    Expression
    Mammalian
    Mutation
    Amino-acids 4127-4303, S4144D
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83458-plasmid-map-sequence-id-159102

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  25. pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)

    Plasmid
    #52134
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-281 and C605S, C631S, C643S …
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549)

    Plasmid
    #50910
    Purpose
    Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V A735C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50910-plasmid-map-sequence-id-76383

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  27. pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)

    Plasmid
    #50899
    Purpose
    Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A720C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50899-plasmid-map-sequence-id-76372

    Enlarge

  28. pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)

    Plasmid
    #50897
    Purpose
    Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L718C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50897-plasmid-map-sequence-id-76370

    Enlarge

  29. pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)

    Plasmid
    #50901
    Purpose
    Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L722C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50901-plasmid-map-sequence-id-76374

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  30. pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)

    Plasmid
    #50895
    Purpose
    Expresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S716C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50895-plasmid-map-sequence-id-76368

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  31. pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)

    Plasmid
    #50900
    Purpose
    Expresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I721C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50900-plasmid-map-sequence-id-76373

    Enlarge

  32. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543)

    Plasmid
    #50907
    Purpose
    Expresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V V729C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50907-plasmid-map-sequence-id-76380

    Enlarge

  33. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)

    Plasmid
    #50908
    Purpose
    Expresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V W732C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50908-plasmid-map-sequence-id-76381

    Enlarge

  34. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547)

    Plasmid
    #50909
    Purpose
    Expresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V W733C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50909-plasmid-map-sequence-id-76382

    Enlarge

  35. pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542)

    Plasmid
    #50906
    Purpose
    Expresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V F728C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50906-plasmid-map-sequence-id-76379

    Enlarge

  36. pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)

    Plasmid
    #50903
    Purpose
    Expresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C722S C723V I724C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50903-plasmid-map-sequence-id-76376

    Enlarge

  37. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)

    Plasmid
    #50904
    Purpose
    Expresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V V726C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50904-plasmid-map-sequence-id-76377

    Enlarge

  38. pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540)

    Plasmid
    #50905
    Purpose
    Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V M727C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50905-plasmid-map-sequence-id-76378

    Enlarge

  39. pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)

    Plasmid
    #50898
    Purpose
    Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    G719C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50898-plasmid-map-sequence-id-76371

    Enlarge

  42. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87829
    Purpose
    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    MTS
    NLS
    PTS1
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87829-plasmid-map-sequence-id-175007

    Enlarge

  49. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

    Plasmid
    #228400
    Purpose
    Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    NES/PKI-mNeonGreen2x-NLS/FUS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228400-plasmid-map-sequence-id-457259

    Enlarge

  50. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

    Plasmid
    #206093
    Purpose
    Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206093-plasmid-map-sequence-id-415677

    Enlarge

Showing: 7201 - 7250 of 12157 results