4,181 results

1. pCMV-lyso-pHoenix

   Plasmid

   #70112

   Purpose

   Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
   

   Tags

   Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-pBAD

   Plasmid

   #54630

   Purpose

   Localization: Protein Expression Vector , Excitation: 587, Emission: 610

   Depositor

   Roger Tsien, Michael Davidson, Nathan Shaner

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Tags

   6xHis and mCherry

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-HA-ER WT

   Plasmid

   #49498

   Purpose

   Expresses HA-tagged ER wild-type in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGTR

   Plasmid

   #63143

   Purpose

   PCR template to construct polycistronic tRNA-gRNA

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   gRNA-tRNA fusion
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-EGFP

   Plasmid

   #89875

   Purpose

   EGFP reporter vector of iTango system for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   tetO-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIO-GFP

   Plasmid

   #74119

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-MMLVgag-3xNES-ABE8e

   Plasmid

   #181751

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-GLIS1

   Plasmid

   #30166

   Purpose

   Retroviral expression of non-canonical human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Maekawa et al Nature. 2011 . 474(7350):225-9.

   Insert

   GLIS1 (GLIS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. DsRed-rab11 DN

   Plasmid

   #12680

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB11A (Homo sapiens) (RAB11A Human)
   

   Tags

   DsRed and HA

   Expression

   Mammalian

   Mutation

   changed serine 25 to asparagine

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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