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qTAG-N-Blast-miRFP670nano3

Plasmid

#207680

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-eArch3.0-mRuby2-ST

Plasmid

#109135

Purpose

Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eArch3.0-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEAK10-His-Myc-Kal9

Plasmid

#25441

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal9

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pXL-BacII-CAG-mCherry

Plasmid

#170519

Purpose

Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-mCherry

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEAK10-His-Myc-Kal5

Plasmid

#25440

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal5

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pBTSG

Plasmid

#159420

Purpose

Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRU47

Plasmid

#167669

Purpose

ENTRY vector for gRNA7 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Blast

Plasmid

#207713

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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phNFL

Plasmid

#132589

Purpose

Expresses human NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLCS109

Plasmid

#209330

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pYN4+

Plasmid

#78715

Purpose

mammalian expression of NHE1

Depositor

Larry Fliegel

Article

Reddy et al J Biol Chem. 2008 Aug 8;283(32):22018-30

Insert

NHE1 (SLC9A1 Human)

Tags

HA3x

Expression

Mammalian

Mutation

D720G

Promoter

RSV-LTR

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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p1242 3x-KB-L

Plasmid

#26699

Depositor

Bill Sugden

Article

Mitchell et al J Virol. 1995 May . 69(5):2968-76.

Insert

3x MHC I KB element - luciferase (NFKB1 Human, Photinus pyralis)

Use

Luciferase

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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pTN14

Plasmid

#178945

Purpose

Expresses AtTIR1(F79G) in C elegans

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

AtTIR1(F74G)

Expression

Worm

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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Human NCKX6-FlagTag (pcDNA3.1+)

Plasmid

#75217

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (SLC8B1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG ARMS

Plasmid

#24086

Depositor

Moses Chao

Article

Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

Insert

ARMS (Kidins220 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pRc/CMV HA TrkB

Plasmid

#39978

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB (Ntrk2 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pOGG001

Plasmid

#113978

Purpose

Level 0 module. nptII promoter for constitutive gene expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

nptII promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pYS28

Plasmid

#202085

Purpose

Parent vector for producing universal minicircle donors for HaloTag C-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

CT Halo-T2A-HygR Minicircle Parent

Use

CRISPR

Tags

HaloTag

Promoter

None

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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IL-4 promoter luciferase

Plasmid

#12195

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-4 promoter (Il4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST14-DoubleCatcher

Plasmid

#216284

Purpose

Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-ER

Plasmid

#139317

Purpose

endoplasmic reticulum-resident FRB for chemically induced dimerization

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-yoHaloTag-TLG1

Plasmid

#105277

Purpose

Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg1 (TLG1 Budding Yeast)

Tags

yoHaloTag

Expression

Yeast

Mutation

HaloTag has yeast optimized codons

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-H2-18 Light chain

Plasmid

#216308

Purpose

Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC115

Plasmid

#216356

Purpose

xNup93-Nb2t Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup93-Nb2t

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-ACTFr

Plasmid

#61192

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-lyn10-mCherry-dLOVPLD

Plasmid

#219556

Purpose

PM-targeted catalytically dead LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-mCherry-dLOVPLD

Tags

mCherry

Expression

Mammalian

Mutation

H170A mutation on PLD

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-p18-mCherry-LOVPLD

Plasmid

#219560

Purpose

lysosome-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

p18-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Article

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Puro

Plasmid

#227262

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Puro-mNeon

Plasmid

#207687

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
RP156 pUAST EGFP:Gr21a

Plasmid

#59524

Purpose

N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Gr21 (Gr21a Fly)

Tags

EGFP

Expression

Insect

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIBA2-Int-SpyTag

Plasmid

#198038

Purpose

Expression plasmid for producing SpyTagged stalled secretion variant of intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

lacks native signal peptide (amino acids 1-39)

Promoter

tet

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-FKBP12-C1

Plasmid

#67900

Purpose

Expresses a fusion of mCherry with FKBP12 in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,506 results for: Emb