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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti-TRE3G-FNLS-PGK-Puro

Plasmid

#110847

Purpose

Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

TRE3G

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LLP339 pGK-dCas9-SunTag

Plasmid

#100943

Purpose

dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

Depositor

Ryan Lister

Publication

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

Plasmid

#100146

Purpose

Expresses CPT1A

Depositor

Peter Howley

Publication

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

CPT1A (CPT1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCW57.1-Luciferase

Plasmid

#99283

Purpose

All-in-one lentivector with tet-inducible luciferase

Depositor

Stephen Tapscott

Publication

Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHAGE2 mCherry-Rtn4a

Plasmid

#86683

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Publication

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRCC-K

Plasmid

#81191

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Publication

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pXJ40 GADD34 Wt (1-674aa)

Plasmid

#75478

Purpose

mammalian expression of GADD34

Depositor

Shirish Shenolikar

Publication

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST-FRT/T0-Flag-PALB2

Plasmid

#71114

Purpose

mammalian expession of FLAG-PALB2

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

Plasmid

#64220

Purpose

Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

Expression

Mammalian

Promoter

CBh and U6

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pST-KT

Plasmid

#44561

Purpose

for ectopic inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Publication

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRcCMV Cep215 (Nigg CW493)

Plasmid

#41152

Depositor

Erich Nigg

Publication

Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cep215 (CDK5RAP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGCDNsam-Hnf4a

Plasmid

#33002

Depositor

Atsushi Suzuki

Publication

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Available since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMX-Gal4-TEAD3

Plasmid

#33106

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TEAD3 (TEAD3 Human)

Tags

Gal4

Expression

Mammalian

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCMX-Gal4-TEAD1

Plasmid

#33108

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TEAD1 (TEAD1 )

Tags

Gal4

Expression

Mammalian

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTRETightBI-RY-0

Plasmid

#31463

Depositor

Phil Sharp

Publication

Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eYFP and mCherry

Tags

NLS

Expression

Mammalian

Available since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pUCBB-eGFP

Plasmid

#32548

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Publication

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Mutation

Codon Optimised wtCNa

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Ago1

Plasmid

#21534

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ago1 (AGO1 Human)

Tags

GFP

Expression

Mammalian

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles