Addgene

We narrowed to 32,144 results for: IND

Showing: 7301 - 7350 of 32144 results

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pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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RFP-HA-DAT

Plasmid

#90265

Purpose

Expresses RFP-HA-DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT)

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMK391 (mAID-BSD)

Plasmid

#121192

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PprgH-GFPLVA

Plasmid

#23340

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Purpose

Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

Depositor

Howard Gu

Article

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Tags

Expression

Mammalian

Mutation

Promoter

Ptet

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 Full Length-6MT

Plasmid

#41728

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Full Length (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

L1997V; Contains murine Notch-1 protein up to ami…

Promoter

Available since

Dec. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-GR11

Plasmid

#89105

Purpose

mammalian expression of full-length human glucocorticoid receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Kupfer et al J Biol Chem. 1993 Aug 15;268(23):17519-2

Insert

human glucocorticoid receptor (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-LOV2

Plasmid

#81009

Purpose

Expression of LOV2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Use

Tags

NTOM20

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV, p10

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-SpCas9

Plasmid

#85450

Purpose

Express SpCas9 in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pRSV

Use

AAV

Tags

Expression

Mutation

Promoter

pRSV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV, p10

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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ART_RTctrl

Plasmid

#214066

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Green Glifon4000

Plasmid

#126208

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon4000

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62 K7A, D69A

Plasmid

#38281

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed K7A, D69A

Promoter

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-puro GFP-p62 dC

Plasmid

#38282

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Truncate mutant 1-265 amino acids residues

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Plasmid

#24418

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-NHERF1

Plasmid

#31650

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Use

Tags

His & S tag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-hISG15

Plasmid

#12447

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15 fragment (ISG15 Human)

Use

Tags

Expression

Mammalian

Mutation

ISG15 up to LRLRGG

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHW110033

Plasmid

#222441

Purpose

Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

Cre recombinase

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pST5552

Plasmid

#36255

Purpose

Mycobacteria replicating vector expressing eGFP.

Depositor

Jessica Seeliger, Carolyn Bertozzi

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Insert

eGFP

Use

Synthetic Biology; Mycobacteria replicating vector

Tags

Expression

Bacterial

Mutation

Promoter

ModifiedHsp60

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TCF1

Plasmid

#40620

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

TCF7 (TCF7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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His-tagged FL Rec8

Plasmid

#45243

Purpose

raise antibody against Rec8

Depositor

Michael Lampson

Article

Chiang et al Curr Biol. 2010 Sep 14;20(17):1522-8. do

Insert

Rec8 (Rec8 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHW110035

Plasmid

#222444

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

LoxP-mCherry-stop

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

no

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCin4-3xFLAG-MKL1

Plasmid

#19838

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2B -1538/+64

Plasmid

#24942

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

Available since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST14-DoubleCatcher-H-Lock

Plasmid

#216285

Purpose

Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher H-Lock

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Use

Tags

FIREmate

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHW101020

Plasmid

#222454

Purpose

Level 1, Position 2 containing 35S::CUP2-p65::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

35S::CUP2-p65::NOS (CUP2 )

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

no

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Green Glifon50

Plasmid

#126206

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon50

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lyn11-targeted FRB (LDR)

Plasmid

#20147

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-linker-FRB (LDR) (MTOR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMU-TPr

Plasmid

#61189

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HaloTag-bActinCDS-bActinUTR-MS2V5

Plasmid

#102718

Purpose

Halo-Actin reporter for detection of both mRNA and protein

Depositor

Robert Singer

Article

Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

HaloTag-bActinCDS-bActin3'UTR-MS2V5

Use

Lentiviral

Tags

HaloTag and MS2V5

Expression

Mutation

Promoter

UbC

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSIIhyg-RGECO1.0

Plasmid

#175813

Purpose

The lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

RGECO1.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
A547 (miR-146a promoter)

Plasmid

#15091

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-GFP-CtIP

Plasmid

#71109

Purpose

mammalian expession of GFP-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMU-PERr

Plasmid

#61202

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-HypaCas9

Plasmid

#190604

Purpose

PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Article

Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHW110031

Plasmid

#222437

Purpose

Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

no

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-4mtD3cpv

Plasmid

#36324

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria

Use

Tags

First 29 aa of human COX subunit 8a

Expression

Mutation

Promoter

CMV

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
hNET pcDNA3

Plasmid

#15475

Depositor

Randy Blakely

Article

Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

Insert

hNET (SLC6A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-SURF4

Plasmid

#170680

Purpose

Expresses FLAG-tagged SURF4 in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

SURF4

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-tdTomato-hphMX6

Plasmid

#105157

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

Insert

tdTomato-hphMX6

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-HHARI

Plasmid

#17450

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

HHARI (ARIH1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
ELP-TEMP/pcDNA3.1(-)

Plasmid

#178434

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

Insert

ELP-TEMP

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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