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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,005 results for: EED

Showing: 2921 - 2940 of 3005 results

  7. psiCHECK-1-miR-376a1(3p)-I-type

    Plasmid
    #215883
    Purpose
    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
    Depositor
    Kumiko Ui-Tei
    Article
    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1
    Insert
    three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    215883-plasmid-map-sequence-id-443531

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  15. Myc-gamma3/gamma2SIL

    Plasmid
    #121433
    Purpose
    GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG3/GABRG2 SIL (Gabrg2, Gabrg3 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    121433-plasmid-map-sequence-id-237864

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  16. Myc-gamma1/gamma2SIL

    Plasmid
    #121432
    Purpose
    GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    121432-plasmid-map-sequence-id-237859

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  17. pCIG1_UL136_33kda_Myc

    Plasmid
    #74957
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Myc tag (EQKLISEEDL)
    Expression
    Mammalian
    Mutation
    M63A M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74957-plasmid-map-sequence-id-141779

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  18. mutpRL-TK 1x perfect to bulged siRNA

    Plasmid
    #40765
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to bulged target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. EF1a_Puro_Telo_v2

    Plasmid
    #195139
    Purpose
    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction
    Depositor
    Jason Sheltzer
    Article
    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11
    Insert
    Telomere (RTEL1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195139-plasmid-map-sequence-id-442720

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Showing: 2921 - 2940 of 3005 results