We narrowed to 18,386 results for: Met

1. gRNA_tdtomato_reporter1_SP-2x

   Plasmid

   #79370

   Purpose

   Assays activity of transcriptional activators fused to SP Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBA328

   Plasmid

   #214748

   Purpose

   Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23119-RBS(22821)-mcherry
   

   Expression

   Bacterial

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46612

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-335-5p Decoy
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. PU6::klp-12_sgRNA

   Plasmid

   #46170

   Purpose

   klp-12 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   klp-12 targeting sgRNA (klp-12 Synthetic)
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDS257

   Plasmid

   #34971

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-4CL1

   Plasmid

   #35947

   Depositor

   Claudia Schmidt-dannert

   Article

   Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

   Insert

   4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLac-mod

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLI-sandwich(0807600)

   Plasmid

   #85790

   Purpose

   P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
   

   Use

   P. falciparum genomic integration

   Tags

   2xFKBP, GFP

   Promoter

   none

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. modRNAc0-Cas9

   Plasmid

   #170180

   Purpose

   IVT template plasmid for making modified Cas9 RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9
   

   Use

   Ivt template plasmid

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

   Plasmid

   #79373

   Purpose

   Recruits VPR to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-VPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AB.pCCL.sin.cPPT.U6.miR-92a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46638

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-92a Decoy
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pTE101

    Plasmid

    #59396

    Purpose

    MethyloBrick cloning vector, no promoter; KmR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    none

    Available Since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. mMaroon-PAK-mMaroon

    Plasmid

    #84361

    Purpose

    FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-PAK-mMaroon
    

    Tags

    mMaroon flanking PAK domain on N and C terminus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKMV-VioD

    Plasmid

    #65350

    Purpose

    systhesis of vioD gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioD
    

    Use

    Synthetic Biology

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEos4a-C1

    Plasmid

    #54810

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mEos4a

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. Gi-c001

    Plasmid

    #173078

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLifeAct_mScarlet-H_N1

    Plasmid

    #85055

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-PhoCl-mCherry-myc

    Plasmid

    #87692

    Purpose

    Express PhoCl-mCherry with C-term myc tag in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl-mCherry-myc
    

    Tags

    myc tag

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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