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pLKO.3G

Plasmid

#14748

Depositor

Christophe Benoist, Diane Mathis

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-KDM4D

Plasmid

#188234

Purpose

Express human KDM4D in mammalian cells

Depositor

Young Tang

Article

Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

Insert

Lysine demethylase 4D (KDM4D Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pME-DreERT2 (JDW 1344)

Plasmid

#224523

Purpose

A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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HA-GbL

Plasmid

#1865

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

GbL (MLST8 Human)

Tags

HA

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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BII-CMV-AfmW3A

Plasmid

#133402

Purpose

Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Afamin and Wnt3a (Afm, Wnt3a Mouse)

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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GW1-PercevalHR

Plasmid

#49082

Purpose

Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Expression

Mammalian

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax_P2A_GFP

Plasmid

#112101

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE9

Plasmid

#90343

Purpose

HIF1a - Hypoxia gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

HIF1a

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-NLS-dCas9-NLS

Plasmid

#99908

Purpose

For the expression of NLS-dCas9-NLS

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Electra2-P2A-EGFP

Plasmid

#184936

Purpose

Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ER-GA

Plasmid

#209871

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRDB_270

Plasmid

#231111

Purpose

C>T base editor

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

TadA-CDd-nCas9-SpG-UGI-HA

Use

CRISPR and Lentiviral

Mutation

D10A

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

Plasmid

#236422

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

KEAP1 (KEAP1 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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SH4-FRB-EBFP2

Plasmid

#222418

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

SH4

Tags

FRB-HA-EBFP2

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Golgi-7

Plasmid

#58018

Purpose

Localization: Golgi Complex, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Golgi (B4GALT1 Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Article

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Mutation

contains 7mer insert PRPPSTH between amino acids …

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pVEC1

Plasmid

#241910

Purpose

Primary screening of PET hydrolases and enzyme condition screening. TphR-based terephthalate (TPA) biosensor

Depositor

Neil Dixon

Article

Alvarez Gonzalez et al Metab Eng Commun. 2024 Nov 6;19:e00250.

Insert

tphR, tphK, sfGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-tmpknot-GG-acceptor

Plasmid

#174039

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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p-dCas9-HDAC1-Hygro

Plasmid

#104409

Purpose

transient expression of dCas9-HDAC1 fusion protein

Depositor

Stephan Beck

Insert

HDAC1 (HDAC1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Hepatitis C Virus NS3/NS4A

Plasmid

#61696

Purpose

inducible dual expression of NS3 protease along with its cofactor NS4A

Depositor

Michael Johnson

Article

Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

Inserts

NS4A
NS3

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3_dCas7-11

Plasmid

#197605

Purpose

Expresses inactive Cas7-11 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

dCas7-11

Expression

Mammalian

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-rAPOBEC1-pAG-hADAR2dd

Plasmid

#248100

Purpose

Expresses rAPOBEC1-pAG-hADAR2dd in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
protein A/G
hADAR2dd (ADARB1 Human)

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-WT-GFP

Plasmid

#187258

Purpose

Lentiviral expression of myosin regulatory light chain-WT

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Promoter

5' LTR

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSM-mStayGold

Plasmid

#234317

Purpose

codon-optimized mStayGold with synthetic introns for the expression in C. elegans

Depositor

Kota Mizumoto

Article

Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

Insert

C. elegans codon optimized mStayGOld

Tags

mStayGold

Expression

Worm

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Plasmid

#177920

Purpose

Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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