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We narrowed to 18,985 results for: INO

Showing: 7351 - 7400 of 18985 results

  5. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid
    #208973
    Purpose
    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208973-plasmid-map-sequence-id-415830

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  10. lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP

    Plasmid
    #202826
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 - two
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    202826-plasmid-map-sequence-id-405885

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  11. lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherry

    Plasmid
    #202821
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete SVA within SVA-lncRNA AK057321 gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (two)
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    202821-plasmid-map-sequence-id-399746

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  12. pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR

    Plasmid
    #202830
    Purpose
    pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    CHAF1B intronic SVA del-VNTR sequence
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    202830-plasmid-map-sequence-id-399708

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  13. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

    Plasmid
    #202825
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 - two
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202825-plasmid-map-sequence-id-399645

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  14. pUt-mNF-GFP

    Plasmid
    #199220
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV D2ir promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199220-plasmid-map-sequence-id-396466

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  20. pHcRed-NPM1cOligodN243-C1

    Plasmid
    #131847
    Purpose
    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection
    Depositor
    Mark Minden
    Article
    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:
    Insert
    NPM1 (NPM1 Human)
    Use
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    This is a deletion contruct of the cytoplasmic m…
    Promoter
    Available since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    131847-plasmid-map-sequence-id-264401

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  21. pHcRed-NPM1cNLSdN243-C1

    Plasmid
    #131848
    Purpose
    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection
    Depositor
    Mark Minden
    Article
    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:
    Insert
    NPM1 (NPM1 Human)
    Use
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    This is a deletion contruct of the cytoplasmic m…
    Promoter
    Available since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    131848-plasmid-map-sequence-id-264370

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  25. S+Q111R+N122D

    Plasmid
    #167175
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+Q111R+N122D
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R and changed N at 122 to D
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167175-plasmid-map-sequence-id-334477

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  26. S+10subs

    Plasmid
    #167176
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+10R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed A at 112 to T; E at 116 to D; I at 135 to…
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167176-plasmid-map-sequence-id-345188

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  27. S+12subs

    Plasmid
    #167177
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+12R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R; A at 112 to T; E at 116to …
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167177-plasmid-map-sequence-id-334485

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  34. MIOX only

    Plasmid
    #166681
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    MIOX (Miox Budding Yeast, Synthetic, Mouse)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166681-plasmid-map-sequence-id-329928

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  38. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  40. EcNGsC-S308P-H309D-pKK223

    Plasmid
    #131378
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera S308P H309D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131378-plasmid-map-sequence-id-259242

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  41. EcNGsC-F307A-S308A-pKK223

    Plasmid
    #131379
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera F307A S308A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are fro…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131379-plasmid-map-sequence-id-259243

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  43. pET-30a(+)-Syk-tSH2-FX

    Plasmid
    #111272
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    interdomain A (Phe 119 to His 162) substituted by…
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111272-plasmid-map-sequence-id-215700

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  44. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92319
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70a
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    92319-plasmid-map-sequence-id-182040

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  45. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92318
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70a
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    92318-plasmid-map-sequence-id-182037

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  46. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

    Plasmid
    #92230
    Purpose
    Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    NTerm 6xHis Tagged zmADH
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue V86 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92230-plasmid-map-sequence-id-181882

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Showing: 7351 - 7400 of 18985 results