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Showing: 7351 - 7400 of 17798 results

  3. pExp-GST

    Plasmid
    #112566
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GST and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112566-plasmid-map-sequence-id-217229

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  4. pEC1.2/EC1.1_spCas9_GFP

    Plasmid
    #161933
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161933-plasmid-map-sequence-id-320699

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  5. pSRKBB-empty

    Plasmid
    #59449
    Purpose
    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.
    Depositor
    Claudia Schmidt-dannert
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    modified lac
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59449-plasmid-map-sequence-id-92571

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  7. SV40-eGFP-Z1

    Plasmid
    #127638
    Purpose
    Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance
    Depositor
    Jordan Green
    Insert
    eGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127638-plasmid-map-sequence-id-248430

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  8. pCW57.1_V5_FLAG_Ct

    Plasmid
    #194075
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    FLAG-V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194075-plasmid-map-sequence-id-381944

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  9. pSNAP-tag (T7) Vector

    Plasmid
    #101137
    Purpose
    Expression of protein of interest with SNAP-Tag in bacterial cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    SNAP-tag (SNAP26b)
    Expression
    Bacterial
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101137-plasmid-map-sequence-id-194162

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  10. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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  14. pDEST-Spe4-Kozak-miRFP713-RfA

    Plasmid
    #186381
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186381-plasmid-map-sequence-id-392698

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  15. pDEST-Spe4-RfA-miRFP713-stop

    Plasmid
    #186391
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186391-plasmid-map-sequence-id-373838

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  16. pmSTARRseq1

    Plasmid
    #96945
    Purpose
    CpG free plasmid for testing effects of methylation on regulatory activity
    Depositor
    Jenny Tung
    Type
    Empty backbone
    Use
    Mstarr-seq screening vector
    Expression
    Mammalian
    Promoter
    CpG -free EF1 promoter
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    96945-plasmid-map-sequence-id-191112

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  17. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid
    #72299
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer
    Available Since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72299-plasmid-map-sequence-id-131901

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  19. pDual_dsCas9_BFP

    Plasmid
    #214679
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214679-plasmid-map-sequence-id-428808

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  20. pCW57.1_TStrp_HA_Ct

    Plasmid
    #194066
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Twin_Strep-HA
    Expression
    Mammalian
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194066-plasmid-map-sequence-id-381940

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  21. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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  22. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid
    #161934
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    161934-plasmid-map-sequence-id-320739

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  24. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pDual_dsCas9_Venus

    Plasmid
    #214678
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214678-plasmid-map-sequence-id-428813

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  27. pDEST-Spe4-RfA-mScarlet-I-stop

    Plasmid
    #186393
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186393-plasmid-map-sequence-id-373587

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  28. pAG GST Empty

    Plasmid
    #236735
    Purpose
    Empty bacterial expression plasmid for GST fusion proteins
    Depositor
    Melina Fan, Addgene Research Program
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Available Since
    April 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236735-plasmid-map-sequence-id-469201

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  29. pMK33-GW-TurboID-V5

    Plasmid
    #196281
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196281-plasmid-map-sequence-id-384554

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  31. pCW57.1_V5_FLAG_Nt

    Plasmid
    #194074
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-FLAG
    Expression
    Mammalian
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194074-plasmid-map-sequence-id-380730

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  32. pCRII-BbsI-sgRNAscaffold

    Plasmid
    #159352
    Purpose
    Expression vector for a customizable guide RNA
    Depositor
    Pim Pijnappel
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159352-plasmid-map-sequence-id-314976

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  34. MZB174

    Plasmid
    #228487
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)-GST-HRV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228487-plasmid-map-sequence-id-455394

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  37. pOpen-T3gene1

    Plasmid
    #165576
    Purpose
    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T3 RNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165576-plasmid-map-sequence-id-340988

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  38. pcDNA3 tdTomato LIC cloning vector (6F)

    Plasmid
    #86963
    Purpose
    Empty LIC vector; adds a tdTomato gene to the C-terminus of your protein of interest.
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86963-plasmid-map-sequence-id-174746

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  42. pDEST-Spe4-RfA-Halotag-stop

    Plasmid
    #186387
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186387-plasmid-map-sequence-id-373586

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  45. pcDNA3 mClover LIC cloning vector (6G)

    Plasmid
    #86964
    Purpose
    Empty LIC vector; adds an mClover gene to the C-terminus of your protein of interest.
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    mClover
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86964-plasmid-map-sequence-id-174657

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  47. pMK33-V5-TurboID-GW

    Plasmid
    #196282
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196282-plasmid-map-sequence-id-384562

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  48. pcDNA3 mRuby2 LIC cloning vector (6H)

    Plasmid
    #86965
    Purpose
    Empty LIC vector; adds an mRuby2 gene to the C-terminus of your protein of interest.
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    mRuby2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86965-plasmid-map-sequence-id-174749

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Showing: 7351 - 7400 of 17798 results