We narrowed to 34,448 results for: CaS

1. pET19b-HisDAD2 cPGFP(G164)

   Plasmid

   #165997

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   HisDAD2 cPGFP(G164)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET19b-rShHTL7 cPGFP(L166)

   Plasmid

   #165995

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rShHTL7 cPGFP(L166)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET19b-DAD2 cPGFP(G164)

   Plasmid

   #165993

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(G164)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEMY07AB-PRO

   Plasmid

   #127710

   Purpose

   Level 0 destination vector for a promoter with A - B scars. Clone in with BpiI

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Fuw CBX8 R19A

   Plasmid

   #121112

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

   Insert

   CBX8 (R19A) (CBX8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   R19A

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Fuw CBX8 R20A

   Plasmid

   #121113

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

   Insert

   CBX8 (R20A) (CBX8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   R20A

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Fuw CBX8 R22A

   Plasmid

   #121114

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

   Insert

   CBX8 (R22A) (CBX8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   R22A

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CeNL(Ca2+)_110μ-pcDNA3

   Plasmid

   #111929

   Purpose

   Cyan color bright luminescent calcium indicator with low affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_110μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E31D, F92W, E104D. D133E at CaM

   Promoter

   CMV

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)

   Plasmid

   #70199

   Purpose

   Promoter panel

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEST-p15A-J23113-deGFP-T500 (124p)

    Plasmid

    #70200

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEST-p15A-pNull-deGFP-T500 (133p)

    Plasmid

    #70209

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEST-p15A-J23102-deGFP-T500 (132p)

    Plasmid

    #70208

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEST-p15A-J23100-deGFP-T500 (130p)

    Plasmid

    #70206

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEST-p15A-J23151-deGFP-T500 (129p)

    Plasmid

    #70205

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEST-p15A-J23106-deGFP-T500 (128p)

    Plasmid

    #70204

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEST-p15A-J23150-deGFP-T500 (127p)

    Plasmid

    #70203

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEST-p15A-J23116-deGFP-T500 (126p)

    Plasmid

    #70202

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEST-p15A-J23114-deGFP-T500 (125p)

    Plasmid

    #70201

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pASKt15C+SrtA5woH

    Plasmid

    #65019

    Purpose

    Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pASKt15C+SrtA2woH

    Plasmid

    #65018

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Promoter

    tetracycline promoter/operator

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEMY07AB-ORF

    Plasmid

    #127711

    Purpose

    Level 0 destination vector for a coding sequence with B - C scars. Clone in with BpiI

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEMY07CD-TER

    Plasmid

    #127712

    Purpose

    Level 0 destination vector for a terminator with C - D scars. Clone in with BpiI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDSM-5a

    Plasmid

    #127716

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 1. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDSM-ab

    Plasmid

    #127717

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 2. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDSM-bc

    Plasmid

    #127718

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 3. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDSM-cd

    Plasmid

    #127719

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDSM-de

    Plasmid

    #127720

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDSM-ef

    Plasmid

    #127721

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDSM-f3

    Plasmid

    #127722

    Purpose

    Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Shh

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Plasmid

    #179115

    Purpose

    Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hsp68

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PB-U6-sgRNA-BFP-Puro

    Plasmid

    #229013

    Purpose

    Perturb Seq sgRNA vector with PiggyBac backbone

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid

    #197511

    Purpose

    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and BPNLS-NpuC(intein)

    Expression

    Mutation

    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

    Promoter

    Cbh

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lenti-sgArid1a#1/Cre

    Plasmid

    #173571

    Purpose

    Expresses a Arid1a-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Arid1a (Arid1a Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid

    #208289

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA; mutations in iSpyMac

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid

    #197507

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDY0750 pCAG-NLS-BceINT

    Plasmid

    #179112

    Purpose

    BceINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

    Plasmid

    #113160

    Purpose

    A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid

    #197508

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

    Promoter

    CMV and T7

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pDY0738 pCAG-NLS-SscINT

    Plasmid

    #179113

    Purpose

    SscINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SscINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pDY0739 pCAG-NLS-SacINT

    Plasmid

    #179114

    Purpose

    SacINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SacINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDY0310 TP901-1 Integrase

    Plasmid

    #179110

    Purpose

    TP901-1 Integrase expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    TP901-1 Integrase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. plentiCRISPRv2-PURO-LYSET-gRNA1

    Plasmid

    #210489

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA1 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. plentiCRISPRv2-PURO-LYSET-gRNA2

    Plasmid

    #210490

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA2 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. Lenti-sgBrca2#1/Cre

    Plasmid

    #173627

    Purpose

    Expresses a Brca2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Brca2 (Brca2 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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