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pAAV-FLEX-SaCas9-U6-sgGria3

Plasmid

#124855

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin1

Plasmid

#124865

Purpose

Mutagenesis of Grin1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin1 (Grin1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA spred1, mitfa:Cas9

Plasmid

#118843

Purpose

Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

spred1 gRNA (spred1 Zebrafish)

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.2-PGK-dTomato

Plasmid

#89605

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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U6-hGRIN2B-CAG-ps-SaCas9

Plasmid

#102853

Purpose

A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. aureus)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Mutation

Promoter

U6 promoter;

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pS23.U6<ActB.mus_C-term-KI_gRNA

Plasmid

#207408

Purpose

gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

ActB

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAs-Cpf1-pGL3-U6-sgRNA

Plasmid

#107683

Purpose

Expresses AsCpf1 crRNA in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLV(shRNA)-Puro-U6-CCEPR

Plasmid

#108277

Purpose

shRNA for CCEPR lncRNA

Depositor

Karl Munger

Article

Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

Insert

CCEPR (CCEPR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TGM2

Plasmid

#69239

Purpose

U6 driven SpCas9 sgRNA expression for TGM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TGM2 sgRNA (TGM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Aa-artsg13 (BsaI)

Plasmid

#188279

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Aa-artsg13

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-biSiRA

Plasmid

#196345

Purpose

Overexpression of circular biSiRA

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

Tornado-biSiRA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

Plasmid

#124870

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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sgPal1 (p2Tol-U6-sgPal1-HygR)

Plasmid

#71483

Purpose

Expresses palindromic sgRNA for use in self-cloning CRISPR

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

sgPal1

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-CB2gRNA-CBh-mCherry

Plasmid

#91948

Purpose

Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6, miniCMV

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGabrg1

Plasmid

#124856

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcnab2

Plasmid

#192795

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcnab2

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

Plasmid

#124864

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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U6>Ebf.774(F+E)

Plasmid

#59990

Purpose

U6 promoter driving Ebf.774 (F+E) sgRNA

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

Ebf.774 (F+E) sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ciinte.U6

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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