Addgene

Showing: 281 - 300 of 2176 results

Results per page:

Sort results by:

p-AAV-sh[SNCA]

Plasmid

#75437

Purpose

knockdown alpha-synuclein in rat neurons

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

snca (Snca Rat)

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218866

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
G protein alpha-s-YFP

Plasmid

#55781

Purpose

This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

alpha-s-EE YFP (Gnas Rat)

Use

Tags

EYFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

Met was substituted for Gln69 in EYFP (Clontech).…

Promoter

CMV

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ERK2

Plasmid

#37145

Depositor

Rony Seger

Article

Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

Insert

ERK2 (MAPK1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa1-6 deleted as a result of cloning strategy

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-DIO-rM3D(Gs)-mCherry

Plasmid

#50458

Purpose

Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

Depositor

Bryan Roth

Has Service

AAV8

Insert

rM3D-mCherry

Use

AAV

Tags

HA and mCherry

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

human Synapsin 1

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRc/CMV HA TrkB

Plasmid

#39978

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB (Ntrk2 Rat)

Use

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p159 pPr-luc

Plasmid

#8392

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin promoter (Pbsn Rat)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-dCpf1-BE

Plasmid

#107685

Purpose

Expresses dLbCpf1-BE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE

Use

Tags

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-hSyn-Axon-jGCaMP8m

Plasmid

#172719

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N2-GRASP65

Plasmid

#137709

Purpose

Eukaryotic expression of rat GRASP65 full-length

Depositor

Yanzhuang Wang

Insert

GRASP65 (Gorasp1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

Plasmid

#105323

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only
p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Expression

Mutation

Promoter

UbC

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-p97

Plasmid

#85670

Purpose

Expression of p97 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

p97 (Vcp Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TGN38 EGFP

Plasmid

#128148

Purpose

Expression of TGN38 amino acids 304-357

Depositor

Jennifer Lippincott-Schwartz

Insert

TGN38 (Tgoln2 Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Contains amino acids 304-357

Promoter

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-OTp-GCaMP6s

Plasmid

#192945

Purpose

To drive GCaMP6s under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-CMV-Galpha-i2 ONE-GO

Plasmid

#189736

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i2 ONE-GO
GNAI2 (Gnai2 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
μ2-HA-WT

Plasmid

#32752

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
Kif5A-CIBN

Plasmid

#102251

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only