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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Publication

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CC10-rtTA

Plasmid

#20179

Depositor

Jeffrey Whitsett

Publication

Stripp et al J Biol Chem. 1992 Jul 25. 267(21):14703-

Insert

rcc10 promoter

Expression

Mammalian

Available since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

Plasmid

#104491

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has service

AAV PHP.eB, AAV Retrograde, and AAV1

Insert

jGCaMP7s

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

Plasmid

#89508

Purpose

Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

Depositor

Jin-Soo Kim

Publication

Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

Insert

rAPOBEC1-nCas9 (D10A)-UGI-NLS

Tags

UGI and rAPOBEC-XTEN

Expression

Bacterial

Mutation

D10A mutation of Cas9

Promoter

T7

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CRY2-GFP-homer1c

Plasmid

#89442

Purpose

Localizes CRY2 to excitatory synapses

Depositor

Matthew Kennedy

Publication

Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

Insert

CRY2-GFP-homer1c

Expression

Mammalian

Mutation

Contains Full length CRY2

Promoter

CAG

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-CMV-DIO-Magneto2.0-sNRPpA

Plasmid

#74307

Purpose

Cre-dependent expression of Magneto2.0 in mammalian cells

Depositor

Ali Guler

Publication

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)

Use

AAV and Cre/Lox

Tags

Flag

Expression

Mammalian

Mutation

TRPV4: delta 760-871

Promoter

CMV (after Cre recombination)

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Publication

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV.hSyn.iGluSnFR3.v857.PDGFR

Plasmid

#178329

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Publication

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has service

AAV1

Insert

pAAV hSyn iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-OMP25

Plasmid

#141150

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

AcGFP

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pORANGE Gria1-GFP KI

Plasmid

#131489

Purpose

Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria1, Gria1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-ArcFL

Plasmid

#119877

Purpose

Bacterial Expression construct for protein production and purification

Depositor

Jason Shepherd

Publication

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Expression

Bacterial

Promoter

tac

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Publication

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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μ2-HA-D176A

Plasmid

#32754

Depositor

Alexander Sorkin

Publication

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Tags

HA

Expression

Mammalian

Mutation

D176A

Promoter

CMV

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Publication

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-IRES-Hygro_GCaMP6s

Plasmid

#164591

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Publication

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCherry

Plasmid

#155222

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b

Tags

mCherry

Expression

Mammalian

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pORANGE Gria2-GFP KI

Plasmid

#131490

Purpose

Endogenous tagging of GluA2: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria2, Gria2 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3G120A

Plasmid

#105003

Purpose

Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Publication

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Glycine 120 mutated to alanine, prevents lipidati…

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Kif5A-GFP-CIBN

Plasmid

#102252

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Publication

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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α1GA-HE3-pcDNA3

Plasmid

#45812

Depositor

Edward Perez-Reyes

Insert

α1Ga (Cacna1g Rat)

Expression

Mammalian

Promoter

CMV

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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