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Plasmid#202462PurposeExpresses redox sensitive GFP (roGFP) after phosphate depletionDepositorInsertreduction-oxidation sensitive green fluorescent protein
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC Destination
Plasmid#207675PurposeTrio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterCymRC, PhlF, VanCCAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHYX137
Plasmid#202814PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX138
Plasmid#202815PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterScADH2Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX143
Plasmid#202816PurposeControl plasmid expressing mScarlet-I fluorescent protein.DepositorInsertmScarlet-I
UseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHYX173
Plasmid#202817PurposePlasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.DepositorInsertTEV protease
UseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN1_EF1a-tNGFR-2A-Puro
Plasmid#200553PurposeDual barcoding cassettes for SSN-seq; Barcode Set 1DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN2_EF1a-tNGFR-2A-Puro
Plasmid#201112PurposeDual barcoding cassettes for SSN-seq; Barcode Set 2DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN3_EF1a-tNGFR-2A-Puro
Plasmid#201113PurposeDual barcoding cassettes for SSN-seq; Barcode Set 3DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN4_EF1a-tNGFR-2A-Puro
Plasmid#201114PurposeDual barcoding cassettes for SSN-seq; Barcode Set 4DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN5_EF1a-tNGFR-2A-Puro
Plasmid#201115PurposeDual barcoding cassettes for SSN-seq; Barcode Set 5DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN6_EF1a-tNGFR-2A-Puro
Plasmid#201116PurposeDual barcoding cassettes for SSN-seq; Barcode Set 6DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN7_EF1a-tNGFR-2A-Puro
Plasmid#201117PurposeDual barcoding cassettes for SSN-seq; Barcode Set 7DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN8_EF1a-tNGFR-2A-Puro
Plasmid#201118PurposeDual barcoding cassettes for SSN-seq; Barcode Set 8DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgTS40
Plasmid#169630PurposepX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.DepositorInsertU6-driven gRNA expression and PCAG-driven SpCas9 expression
UseTagsExpressionMammalianMutationPromoterU6 / PCAGAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1041
Plasmid#169619PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH228-Tier1-OVanO2-PCMVmin2-SEAP
Plasmid#169564PurposeTier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).DepositorInsertvanillic acid-controlled SEAP production
UseTagsExpressionMammalianMutationPromoterVanO2-PCMVminAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1040
Plasmid#169618PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1039
Plasmid#169617PurposeTier-2 vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP
Plasmid#169588PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-2Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH63-Tier1-PMLP-Citrine_p2a_SEAP
Plasmid#169557PurposeTier-1 vector encoding PMLP-driven Citrine-p2A-SEAP expression (PMLP-Citrine-p2A-SEAP-pA).DepositorInsertPMLP-driven citrine-P2A-SEAP fusion construct
UseTagsExpressionMammalianMutationPromoterPMLPAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2366-Tier1-PhCMV_F-type
Plasmid#169567PurposeTier-1 vector encoding PhCMV-driven F-type transactivator domain (PhCMV-F-type-pA).DepositorInsertPCMV-driven F-type VP16
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH255-Tier1-OTetO7-PCMVmin1-SEAP
Plasmid#169587PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-1Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH65-Tier1-PTK-Citrine_p2a_SEAP
Plasmid#169559PurposeTier-1 vector encoding PTK-driven Citrine-p2A-SEAP expression (PTK-Citrine-p2A-SEAP-pA).DepositorInsertPTK-driven citrine-P2A-SEAP fusion construct
UseTagsExpressionMammalianMutationPromoterPTKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2388-Tier1-PhCMV-p2a_Ypet
Plasmid#169525PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).DepositorInsertPCMV-driven P2A-fused yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2387-Tier1-PhCMV-p2a_mTagBFP2
Plasmid#169524PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).DepositorInsertPCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2389-Tier1-PhCMV-p2a_mRuby
Plasmid#169526PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).DepositorInsertPCMV-driven P2A-fused enhanced yellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH321-1-Tier1-PhCMV-dCas9-3xNLS
Plasmid#169595PurposeTier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).DepositorInsertdead S.pyogenes Cas9
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2385-Tier1-PhCMV-fastFT
Plasmid#169518PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).DepositorInsertPCMV-driven fluorescent timer, fast maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2384-Tier1-PhCMV-medFT
Plasmid#169517PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).DepositorInsertPCMV-driven fluorescent timer, medium maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2408-Tier1-PhCMV-TEVsite
Plasmid#169503PurposeTier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).DepositorInsertPCMV-driven tobacco etch virus protease cleavage site
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2405-Tier1-PhCMV-TEV
Plasmid#169500PurposeTier-1 vector encoding PhCMV-driven tabacco etch virus protease TEV (PhCMV-TEV-pA).DepositorInsertPCMV-driven tobacco etch virus protease
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR
Plasmid#169499PurposeTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)DepositorInsertPRPBSA-driven YPet-P2aA-PuroR
UseTagsExpressionMammalianMutationPromoterPRBSAAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1047-Tier1-PhCMV-YPet-p2A-PuroR
Plasmid#169498PurposeTier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).DepositorInsertPCMV-driven YPet-P2aA-PuroR
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1023-Tier1-PhCMV-p2A-PuroR
Plasmid#169497PurposeTier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).DepositorInsertPCMV-driven P2A-PuroR
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH9-Tier1-PhCMV-3xNLS
Plasmid#169478PurposeTier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).DepositorInsertPCMV-driven nuclear localization signal
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-pntAB
Plasmid#158609PurposeLow phosphate induction of pntABDepositorInsertpntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
UseTagsExpressionBacterialMutationPromoterInsulated ugpBp from E. coliAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC031
Plasmid#171152PurposepGNW2 derivative with integration site at P. putida prophage1 for integration of J3-BBa_J23117-mRFP cassetteDepositorInsertJ3-BBa_J23117-mRFP
UseTagsExpressionBacterialMutationPromoterJ3-BBa_J23117Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC034
Plasmid#171153PurposepGNW2 derivative with integration site at P. putida prophage2 for integration of J3(106)-BBa_J23111-sfGFP cassetteDepositorInsertJ3(106)- BBa_J23111-sfGFP
UseTagsExpressionBacterialMutationPromoterJ3(106)-BBa_J23111Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC018.AAV
Plasmid#171146PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC016.109
Plasmid#171144PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG
Plasmid#158132PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG2
Plasmid#158134PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the second serine residu…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG3
Plasmid#158136PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third alanine residu…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG4
Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPJ1
Plasmid#83561PurposeExpress yEGFP-ERG9 ER signal peptide under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER signal peptide
UseTagsExpressionYeastMutationPromoterTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW022
Plasmid#83532Purposepheromone mediated expression of UBiC, ARO4, and TKL1DepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW015
Plasmid#83530PurposeUBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoterDepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW018
Plasmid#83531PurposeAromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpinDepositorInsertpARO9-mf_2-pFUS1J2-ARO7i-CYC1t
UseTagsExpressionYeastMutationPromoterpARO9/pFUS1J2Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas59
Plasmid#82396PurposesgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only