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We narrowed to 13,975 results for: SHI

Showing: 701 - 750 of 13975 results
  1. ATG-1929

    Plasmid
    #108712
    Purpose
    CBR2opt in pF4Ag expression vector with T7 and CMV promoter
    Depositor
    Insert
    CBR2opt
    Expression
    Bacterial and Mammalian
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    T7, CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pAC149-pCR8-dCas9VP160

    Plasmid
    #48221
    Purpose
    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP160
    Mutation
    D10A;H840A nuclease-deficient
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
  3. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available Since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
  4. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid
    #176461
    Purpose
    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV
    Depositor
    Insert
    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pDONRG_P4-P1R:MUM4_0.3Pro_35S

    Plasmid
    #128558
    Purpose
    Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    Gateway promoter entry clone
    Promoter
    MUM4 core promoter with 35S enhancer
    Available Since
    Sept. 25, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. LentiV_Neo_SIK3_CR_T221A

    Plasmid
    #108107
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene
    Depositor
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid
    #123343
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Insert
    Venus-cpVenus-FLARE-CKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. LentiV_Neo_SIK3_CR_T142Q

    Plasmid
    #108109
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene
    Depositor
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 701 - 750 of 13975 results