We narrowed to 814 results for: control shrna

1. pLentiCRISPRv2_mSTING_scrambled_gRNA_1

   Plasmid

   #196627

   Purpose

   Scrambled control 1 for STING knock-out in murine cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING scrambled gRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiCRISPRv2_mSTING_scrambled_gRNA_2

   Plasmid

   #196628

   Purpose

   Scrambled control 2 for STING knock-out in murine cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING scrambled gRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDECKO_mCherry_non-targeting

   Plasmid

   #140109

   Purpose

   CRISPR-Cas9 library validation (negative control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. XLone-Puro Cas13d-eGFP U6 SOX17 g2

   Plasmid

   #155188

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   Cas13d SOX17 gRNA2
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AA290

   Plasmid

   #215949

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD55_v4; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AA286

   Plasmid

   #215948

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD47_v6; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDECKO_mCherry_Rat_cebp-alfa

   Plasmid

   #140098

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDECKO_mCherry_SPI1

   Plasmid

   #140099

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJMP1341

   Plasmid

   #119272

   Purpose

   control sgRNA in cells lacking rfp

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-EF1a-Ly6G-miR30

    Plasmid

    #163358

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMY-Ly6G-Rluc_miR

    Plasmid

    #163339

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid

    #241026

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven empty sgRNA
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SAM-DNMT3A-inactive+sgGFP

    Plasmid

    #213169

    Purpose

    Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgGFP
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa and U6

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSuper Retro GFP DGK zeta mouse

    Plasmid

    #224577

    Purpose

    Negative Control for downregulation of the expression of human DGK zeta in human cells

    Depositor

    Isabel Mérida

    Article

    Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

    Insert

    sh RNAi Diacylglycerol kinase zeta mouse (Dgkz Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AA390

    Plasmid

    #215957

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control reporter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgEGFP_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AA246

    Plasmid

    #215946

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD55_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AA233

    Plasmid

    #215941

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA244

    Plasmid

    #215945

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA242

    Plasmid

    #215943

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA243

    Plasmid

    #215944

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v5 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AA310

    Plasmid

    #215950

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control lethal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AA232

    Plasmid

    #215940

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AA238

    Plasmid

    #215942

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v4 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AA285

    Plasmid

    #215947

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD81_v1; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AA021

    Plasmid

    #215929

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs];
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AA023

    Plasmid

    #215931

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXPR_BRD003 Ch2-2

    Plasmid

    #184486

    Purpose

    Lentivirus gRNA targeting Ch2-2 (control)

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    Ch2-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

    Plasmid

    #168243

    Purpose

    "Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Rac(guide resistant)-DN
    

    Use

    Zebrafish expression

    Tags

    mcherry

    Expression

    Mutation

    Promoter

    LyzC

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XLone-Puro Cas13d-eGFP U6 RUNX1 g1

    Plasmid

    #155185

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d RUNX1 gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. XLone-Puro Cas13d-eGFP U6 RUNX1 g2

    Plasmid

    #155186

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d RUNX1 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. XLone-Puro Cas13d-eGFP U6 SOX17 g1

    Plasmid

    #155187

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA1

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDECKO_mCherry_Intergenic

    Plasmid

    #140100

    Purpose

    CRISPR-Cas9 library validation (negative control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-sgCTRL-1

    Plasmid

    #83934

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-sgCTRL-2

    Plasmid

    #83935

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-sgCTRL-3

    Plasmid

    #83936

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-EF1a-CD90.2-Rluc_miR

    Plasmid

    #163360

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pdCas9-DNMT3A-PuroR_hNT

    Plasmid

    #71830

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    Non-targeting sgRNA human (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid

    #154197

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC2-A, sgRNA-BC2-C
    

    Use

    Lentiviral; Catch barcode activation

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and mU6

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid

    #71828

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid

    #187459

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMCB320-sgNC.SPA

    Plasmid

    #169834

    Purpose

    Expresses a negative control sgRNA

    Depositor

    Laura Attardi

    Article

    Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

    Insert

    safe harbor sgRNA
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LentiCas9-sgCh2-2-Blast

    Plasmid

    #199645

    Purpose

    Expresses Cas9 and sgRNA control guide targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti-SwitchON-sgCh2-2

    Plasmid

    #199637

    Purpose

    Tamoxifen-inducible expression of sgRNA control targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. sgCTRL-hygro

    Plasmid

    #199639

    Purpose

    sgRNA control; induces CAS9 cutting in an intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129048

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129041

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMCB320-sgNC.ACOCA

    Plasmid

    #169836

    Purpose

    Expresses a negative control sgRNA

    Depositor

    Laura Attardi

    Article

    Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

    Insert

    safe harbor sgRNA
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid

    #163359

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMY-Ly6G-P2A-PuroR-HA-Rluc_miR

    Plasmid

    #163340

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRDA052H_sgCh2

    Plasmid

    #229023

    Purpose

    Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge