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We narrowed to 772 results for: tev cleavage

Showing: 701 - 750 of 772 results

  5. TLNRD1(4H)-2E_pET151

    Plasmid
    #224068
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS; V5
    Expression
    Bacterial
    Mutation
    K192E/R233E double mutant; prevents actin binding…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224068-plasmid-map-sequence-id-443085

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  8. ATX3-JD

    Plasmid
    #184247
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain (ATXN3 Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon V183X
    Promoter
    T7 promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184247-plasmid-map-sequence-id-370313

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  18. Clomeleon

    Plasmid
    #203723
    Purpose
    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration
    Depositor
    George Augustine
    Article
    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1
    Insert
    cyan fluorescent protein linked yellow florescent protein
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Cyan Fluorescent Protein, Topaz Fluorescent Prote…
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203723-plasmid-map-sequence-id-403632

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  20. hTln1-R12DD-SCLS

    Plasmid
    #191443
    Purpose
    expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397
    Depositor
    Ben Goult
    Article
    Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.
    Insert
    Human Talin1 R12DD (TLN1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    deletion between residues K2375 and A2397
    Promoter
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191443-plasmid-map-sequence-id-380513

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  21. hTln1-R1R2-17b

    Plasmid
    #191427
    Purpose
    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria
    Depositor
    Ben Goult
    Article
    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901
    Insert
    Human Talin1 R1R2 domains (TLN1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    contains 17AA insertion from inclusion of exon 17b
    Promoter
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    191427-plasmid-map-sequence-id-380511

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  25. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

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  28. pFBOH-MHL_SHKBP1:182-585

    Plasmid
    #210935
    Purpose
    Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    SHKBP1:T182-L585 (SHKBP1 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    Q507L natural variant (VAR_036714)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210935-plasmid-map-sequence-id-426241

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  36. pFBOH-MHL_WDR13:142-485

    Plasmid
    #210924
    Purpose
    Insect expression of human WDR13 fragment A142 to K485. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR13:A142-K485 (WDR13 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    H325R natural variant (VAR_060284)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210924-plasmid-map-sequence-id-426206

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  41. pFBOH-MHL_WDR12:90-417

    Plasmid
    #210903
    Purpose
    Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR12:T90-T417 (WDR12 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    introduced mutations F99A K131A R319S
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210903-plasmid-map-sequence-id-426133

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  49. pNICBio2_WDR5:2-334

    Plasmid
    #211027
    Purpose
    bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR5:A2-C334 (WDR5 Human)
    Use
    Tags
    MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE
    Expression
    Bacterial
    Mutation
    wild type
    Promoter
    T7
    Available since
    April 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    211027-plasmid-map-sequence-id-426277

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Showing: 701 - 750 of 772 results