We narrowed to 1,551 results for: MBP

1. p2CT-His-MBP-Lwa_Cas13a_WT

   Plasmid

   #91865

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LwaCas13a
   

   Use

   CRISPR

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-CHC(1-1074)-His6

   Plasmid

   #100748

   Purpose

   Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CLTC (CLTC Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Residues 1-1074

   Promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-6His-MBP-MpAgo

   Plasmid

   #79249

   Purpose

   This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

   Depositor

   Jennifer Doudna

   Article

   Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

   Insert

   Marinitoga piezophila Argonaute
   

   Use

   CRISPR

   Tags

   6XHis-tag + MBP + PreScission Cleavage Site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6His-MBP-TEV-huAsCpf1

   Plasmid

   #90095

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huAsCpf1
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6His-MBP-TEV-huLbCpf1

   Plasmid

   #90096

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huLbCpf1
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-TSF-TEVcs-ULK1K46I

   Plasmid

   #171417

   Purpose

   For the purifcation of human ULK1 kinase dead complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ULK1 (ULK1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   kinase dead

   Promoter

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET41-MBP-t-A3A-t-His

   Plasmid

   #109231

   Purpose

   Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

   Depositor

   Rahul Kohli

   Article

   Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

   Insert

   human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-AviTag-DogTag-MBP

   Plasmid

   #171773

   Purpose

   Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogTag-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pExp-xMBP-ACE2-CHis

   Plasmid

   #194998

   Purpose

   Production of ACE2 extracellular domain

   Depositor

   Marko Hyvönen

   Article

   Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   His8 and xMBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKEW189-MBP-TEV-AcrVA1

    Plasmid

    #115660

    Purpose

    Expresses AcrVA1 in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA1
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_MBP-RGG-GFP-RGG

    Plasmid

    #124949

    Purpose

    Expression of MBP-RGG-GFP-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    EGFP, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB-His-MBP-SARM1

    Plasmid

    #195762

    Purpose

    Purification of SARM1 from E.coli

    Depositor

    Hao Wu

    Insert

    SARM1 (SARM1 Human)
    

    Use

    Tags

    HIS-MBP-TEV

    Expression

    Bacterial

    Mutation

    truncation of first 19 residues

    Promoter

    T7

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC102-His-MBP-DDX3X(WT)

    Plasmid

    #219988

    Purpose

    DDX3X(WT) protein purification

    Depositor

    Simon Alberti

    Insert

    DDX3X(WT) (DDX3X Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-FIP200(1-640)-MBP

    Plasmid

    #203543

    Purpose

    FIP200 NTD-GFP mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    MBP-10His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2CT-His-MBP-Lse_C2c2_WT

    Plasmid

    #83486

    Purpose

    Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lse_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-His6MBP-A3A-Gly6His6

    Plasmid

    #187822

    Purpose

    Expression human APOBEC3A in E.coli.

    Depositor

    Hao Wu

    Article

    Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

    Insert

    APOBEC3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter (on the backbone)

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2CT-His-MBP-Lba_Cas13a_WT

    Plasmid

    #91867

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbaCas13a (LbaC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2CT-His-MBP-Lsh_C2c2_WT

    Plasmid

    #83487

    Purpose

    Bacterial expression of WT L. shahii C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lsh_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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