We narrowed to 296 results for: nisch

1. cTRE-PtenC124S

   Plasmid

   #135664

   Purpose

   Introduce Dox-inducible (TREa promoter) C124S-mutant Pten cDNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   Pten (Pten Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   C124S

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetO-FUW-pgk-puro

   Plasmid

   #85747

   Purpose

   tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

   Depositor

   Emily Dykhuizen

   Article

   Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   none

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-RLuc-T2A-PuroR

   Plasmid

   #102320

   Purpose

   expression of r-luciferase

   Depositor

   Rudolf Jaenisch

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   Renilla luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ubc

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mGDF8 donor mCherry-PA

   Plasmid

   #72596

   Purpose

   Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   mCherry-puromycin cassetter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-Puro_siKD

   Plasmid

   #86695

   Purpose

   Tetracycline inducible expression of shRNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 TO

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetO-FUW-PBRM1-pgk-puro

   Plasmid

   #85746

   Purpose

   tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

   Depositor

   Emily Dykhuizen

   Article

   Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAGGS-mNAGA

   Plasmid

   #196448

   Purpose

   Expresses modified α-N-acetylgalactosaminidase in mammalian cells

   Depositor

   Yuichiro Miyaoka

   Article

   Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

   Insert

   modified alpha-N-acetylgalactosaminidase (NAGA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-TLR targeting vector

   Plasmid

   #64215

   Purpose

   Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Insert

   traffic light reporter (TLR) (AAVS1 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   see publication for details

   Promoter

   CAG

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mGDF8 donor CFP-hDF

   Plasmid

   #72590

   Purpose

   Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. hGDF8 donor CFP-hDF

    Plasmid

    #72591

    Purpose

    Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. FOR human OCT4 TALEN

    Plasmid

    #52412

    Purpose

    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    HD NG NN NN NN HD NG HD NG HD HD HD NI NG
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

12. REV human OCT4 TALEN

    Plasmid

    #52413

    Purpose

    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pDonor-FMR1-dCGG0

    Plasmid

    #222965

    Purpose

    To create 0CGG FMR1 cell line

    Depositor

    Xinyu Zhao

    Article

    Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

    Insert

    FMR1 homologous template no CGG repeats (FMR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    CGG repeats deleted from 5' UTR

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDonor-FMR1-CGG-BsmBI

    Plasmid

    #222966

    Purpose

    0CGG with BsmBI site for inserting CGG repeats

    Depositor

    Xinyu Zhao

    Article

    Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

    Insert

    FMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    CGG repeats deleted from 5' UTR, BsmBI site …

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Px330-EGFP-LRRK2-CRISPR/Cas9

    Plasmid

    #180437

    Purpose

    Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    gRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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