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Addgene
Showing: 281 - 300 of 1002 results

  1. BII-ChPtW-tdT

    Plasmid
    #133401
    Purpose
    Piggybac vector for constitutive expression of tdTomato
    Depositor
    Blair Madison
    Insert
    tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Availability
    Academic Institutions and Nonprofits only
    133401-plasmid-map-sequence-id-268578

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  2. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Mutation
    Promoter
    TetO Promoter
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

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  3. PB-TAC-OK+9MS-Cas9

    Plasmid
    #120354
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120354-plasmid-map-sequence-id-246101

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  4. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid
    #120357
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120357-plasmid-map-sequence-id-236025

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  6. pEF1α-M2rtTA-T2A-Puro

    Plasmid
    #169175
    Purpose
    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174
    Depositor
    Igor Slukvin
    Article
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    M2rtTA-T2A-Puro
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor Comments Below
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    169175-plasmid-map-sequence-id-362474

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  12. PB-UBC-GCAMP6f-PGK-neo

    Plasmid
    #160049
    Purpose
    expression of GCaMP6f in a piggybac plasmid
    Depositor
    Xiaojun Lian
    Insert
    GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ubc
    Availability
    Academic Institutions and Nonprofits only
    160049-plasmid-map-sequence-id-313848

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  13. BII-tdT-inv-GFP

    Plasmid
    #133393
    Purpose
    Piggybac vector for bidirectional promoter reporter assay
    Depositor
    Blair Madison
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    No promoter
    Availability
    Academic Institutions and Nonprofits only
    133393-plasmid-map-sequence-id-268036

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  14. BII-STAR-tdT

    Plasmid
    #133396
    Purpose
    Piggybac vector for ASCL2 reporter
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Stem Cell Specific ASCL2 response elements with L…
    Availability
    Academic Institutions and Nonprofits only
    133396-plasmid-map-sequence-id-268580

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  15. BII-ChPtW-iRFP702

    Plasmid
    #133398
    Purpose
    Piggybac vector for constitutive expression of iRFP702
    Depositor
    Blair Madison
    Insert
    IRFP-702
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Availability
    Academic Institutions and Nonprofits only
    133398-plasmid-map-sequence-id-267650

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  16. BII-ChPtW-smURFP

    Plasmid
    #133399
    Purpose
    Piggybac vector for constitutive expression of smURFP-NLS
    Depositor
    Blair Madison
    Insert
    smURFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Availability
    Academic Institutions and Nonprofits only
    133399-plasmid-map-sequence-id-268584

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  17. pSARGENT1

    Plasmid
    #216315
    Purpose
    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence
    Depositor
    Barak Cohen
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    216315-plasmid-map-sequence-id-430346

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  18. pPB-CAG-rtTAM2-IB

    Plasmid
    #214117
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    rtTAM2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    214117-plasmid-map-sequence-id-423608

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  19. pPB-CAG-rtTA3G-IH

    Plasmid
    #214118
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    trTA3G
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    214118-plasmid-map-sequence-id-423610

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  20. PB-NEO-GFP-RFP

    Plasmid
    #157952
    Purpose
    seamless gene editing and component of Piggybac transposase system
    Depositor
    Yan-zhen Bi
    Type
    Empty backbone
    Use
    Mouse Targeting; Gene targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    157952-plasmid-map-sequence-id-310668

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Showing: 281 - 300 of 1002 results