We narrowed to 14,457 results for: EGF

1. pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

   Plasmid

   #218635

   Purpose

   Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   N1008R, R1009E, E1010R, R1013E

   Promoter

   UBC

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S

   Plasmid

   #147515

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A (gw Human, Nematode)
   

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R

   Plasmid

   #147389

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Fly, Nematode)
   

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EK0494 SFFV-mCherry-SGc(CFL1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191181

   Purpose

   Expression of CFL1 sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:CFL1.s1:EGFP-3xMS2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

   Plasmid

   #191166

   Purpose

   Mammalian expression of a EGFP mutant N213D derived from EK0379.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   EGFP(N213D)
   

   Expression

   Mammalian

   Mutation

   c.637A>G(N213D; CCA>CCG)

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

   Plasmid

   #185489

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

   Promoter

   CMV and T7

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

   Plasmid

   #185492

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV and T7

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R

   Plasmid

   #147394

   Purpose

   Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Human, Nematode)
   

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

   Plasmid

   #176139

   Purpose

   EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   XRCC1-T2A-PolB (XRCC1, POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP and MYC

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide4

    Plasmid

    #125443

    Purpose

    CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide3

    Plasmid

    #125442

    Purpose

    CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide2

    Plasmid

    #125441

    Purpose

    CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hPGK and U6

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190034

    Purpose

    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Expression

    Mammalian

    Mutation

    A134V,A140V,A141V,A143V

    Promoter

    CBh, SV40, hU6

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Mutation

    DR1 A>T

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85006

    Purpose

    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60231

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Promoter

    U6 and hSyn

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Plasmid

    #179116

    Purpose

    Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site for in-frame fusion
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60230

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Promoter

    U6 and hSyn

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR

    Plasmid

    #235204

    Purpose

    Plasmids for constructing mitoBEs v2

    Depositor

    Wensheng Wei

    Article

    Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR
    

    Expression

    Mammalian

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033)

    Plasmid

    #214852

    Purpose

    Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs)

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

    Plasmid

    #205540

    Purpose

    Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TRE3GS

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LSM081 SFFV-mCherry-SGc(stem-1(3xMS2)2-stops)cSG-EGFP (FLP-IN)

    Plasmid

    #233440

    Purpose

    SFFV driven LIDAR reporter with an additional stop codon on the stem loop

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    SGc(stem-1(3xMS2)2-stops)cSG-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

    Plasmid

    #86230

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    AID[71-114]-eGFP
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035)

    Plasmid

    #214853

    Purpose

    Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs)

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

    Plasmid

    #45789

    Depositor

    Richard Murray

    Inserts

    deGFP
    Tetracycline repressor
    araC
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid

    #92140

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    AID[71-114]-eGFP
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid

    #208290

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations and V106W in TadA and nSpCas9(D10…

    Promoter

    CMV and T7

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AiP15579 - pAAV-AiE0780m-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5579)

    Plasmid

    #224201

    Purpose

    AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #218634

    Purpose

    Proximity biotinylation of msNLRP3(ΔPYD, I125M-END)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    delPYD (I125M-END)

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188901

    Purpose

    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    SID-dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AiP15148: pAAV-AiE0779m_6xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5148)

    Plasmid

    #214870

    Purpose

    Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs)

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AiP15457 - pAAV-AiE0441h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5457)

    Plasmid

    #224198

    Purpose

    AiE0441h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal MSNs

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid

    #156452

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG

    Depositor

    Elphege Nora

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    RAD21, AID (Rad21 Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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36. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid

    #176461

    Purpose

    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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37. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid

    #185488

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid

    #185485

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA

    Plasmid

    #246436

    Purpose

    Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA with trans-splicing template
    

    Tags

    GFP N-terminal sequence (M1 to Q95), Hemi Intron …

    Expression

    Mammalian

    Mutation

    GFP N-terminal sequence M1 to Glutamine 95 append…

    Promoter

    CMV and U1a

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NSK242 SFFV-mCherry-SGc(os13.NOT-SMN2.36,55,36)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247283

    Purpose

    modulADAR sensor for NOT SMN exon 7

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-os13.NOT-SMN2.36,55,36-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. NSK185 SFFV-mCherry-SGc(EBER1.os13.8)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247280

    Purpose

    modulADAR sensor for EBER1

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-EBER1.os13.8-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. EK0716 SFFV-mCherry-SGc(s70587.os18)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247271

    Purpose

    modulADAR sensor against sythetic target transcript (35nt GluR-B stemloop)

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-s70587.os18-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. NSK267 SFFV-mCherry-SGc(EBER2.linear.1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247288

    Purpose

    Linear sensor for EBER2

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-os13.EBER2.linear.1-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. NSK266 SFFV-mCherry-SGc(EBER1.linear.2)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247287

    Purpose

    Linear sensor for EBER1

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-os13.EBER1.linear.2-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. EK0698 SFFV-mCherry-SGc(s70587.os13)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247270

    Purpose

    modulADAR sensor against sythetic target transcript (45ntGABRA3 stemloop)

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-s70587.os13-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EK0718 SFFV-mCherry-SGc(s70587.os20)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247273

    Purpose

    modulADAR sensor against sythetic target transcript (36nt GABRA3 stem-loop)

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-s70587.os20-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. EK0717 SFFV-mCherry-SGc(s70587.os19)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247272

    Purpose

    modulADAR sensor against sythetic target transcript (41nt GluR-B stemloop)

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-s70587.os19-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. NSK195 SFFV-mCherry-SGc(EBER2.os13.9)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247281

    Purpose

    modulADAR sensor for EBER2

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-EBER2.os13.9-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. NSK241 SFFV-mCherry-SGc(os13.NOT-SMN2.45,55,45)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #247282

    Purpose

    modulADAR sensor for NOT SMN exon 7

    Depositor

    Xiaojing Gao

    Article

    Kolber et al bioRxiv 2025.09.18.677213

    Insert

    mCherry-os13.NOT-SMN2.45,55,45-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCMV-Frankenbody-HA-mScarlet3-tdP2A-Frankenbody-FLAG-mEGFP-IRESv4-HaloTag-tdMCP

    Plasmid

    #241140

    Purpose

    Expresses Anti-FLAG frankenbody-mScarlet3, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent HA and FLAG-tagged proteins and MS2-tagged mRNA

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-FLAG frankenbody
    anti-FLAG frankenbody
    tdMCP
    

    Tags

    HaloTag, mEGFP, and mScarlet3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge