We narrowed to 7,181 results for: RAP

1. pH6HTC_FAM98A-Halo

   Plasmid

   #175329

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   FAM98A (FAM98A Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pThermeleon

   Plasmid

   #86120

   Purpose

   Bandpass temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   TcI38
   mCherry
   cI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

   Promoter

   pR/pL, pR/pL, pLacI, and pTlpA

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CFP-FKBP (CF)

   Plasmid

   #20160

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   CFP-FKBP (FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-MRGBP

   Plasmid

   #15325

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

   Insert

   MRG-binding protein (MRGBP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pTP341 pHAGE2 CMV TagBFP-3xFLAG

   Plasmid

   #199391

   Purpose

   Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   TagBFP-3xFLAG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPP381

   Plasmid

   #158798

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   RuvC-inactivating mutation D394A

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV U6+27

   Plasmid

   #25709

   Purpose

   for nuclear expression of small RNAs in mammalian cells

   Depositor

   David Engelke

   Article

   Good et al Gene Ther. 1997 Jan . 4(1):45-54.

   Insert

   U6 snRNP (LSM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Clover-Prx2-mRuby2

   Plasmid

   #117906

   Purpose

   fluorescent reporter of cytosolic hydrogen peroxide

   Depositor

   Hadley Sikes

   Article

   Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

   Insert

   Clover-Prx2-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rogi2-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179865

   Purpose

   Targets Rogi2 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SB_loop

    Plasmid

    #110103

    Purpose

    pBXPHM3 with scaffold Sybody of the loop library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the loop library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Sirius-mT-Sapphire_pcDNA3

    Plasmid

    #89584

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

    Insert

    Sirius-mT-Sapphire
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL2399

    Plasmid

    #137051

    Purpose

    Expression of rapamycin-inducible dimerisation of split Cre from ribosomal locus in Leishmania parasite; pRIB-DiCre

    Depositor

    Jeremy Mottram

    Article

    Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

    Inserts

    Split Cre Recombinase Rapamycin Inducible Dimerisation
    Blasticidin-S Deaminase
    

    Use

    Cre/Lox; Leishmania ribosomal locus expression

    Tags

    Expression

    Mutation

    Promoter

    For integration into the 18S ribosomal locus

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SB_convex

    Plasmid

    #110104

    Purpose

    pBXPHM3 with scaffold Sybody of the convex library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the convex library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-CaM-V5-sTb(N)

    Plasmid

    #219779

    Purpose

    sTb(N) half of CaST for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CaM-V5-sTb(N)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-CD4-sTb(C)-M13-GFP

    Plasmid

    #219780

    Purpose

    sTb(C) half CaST for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTb(C)-M13-GFP
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mCherry-Zdk1-TIAM1 DH/PH

    Plasmid

    #81129

    Purpose

    mammalian expression of Zdk1 tagged Tiam1 Dh/PH

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    TIAM1 DH/PH (TIAM1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGal4AD-CIBN

    Plasmid

    #28246

    Purpose

    Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1 (1-171aa)
    

    Use

    Tags

    Gal4AD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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