We narrowed to 6,879 results for: nsf

1. NbPT5b-p3

   Plasmid

   #160001

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   Bar (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Agrobacterium tumefaciens Nopaline synthase Promo…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NbBCP1b-p3

   Plasmid

   #160002

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbBCP1b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   Bar (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Agrobacterium tumefaciens Nopaline synthase Promo…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCL-PGK-SPdCas9-BFP-DNMT3A

   Plasmid

   #66819

   Purpose

   dCas9 fused to BFP and the human DNMT3A catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
   

   Tags

   dSpCas9-BFP-DNMT3A

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDN-D2irC6kwh

   Plasmid

   #44725

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   mCherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA

   Plasmid

   #218795

   Purpose

   AAV reporter genome plasmid

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   CMV-CBA-hGBA1-cm-hGBA1-hGHpA
   

   Use

   AAV

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-Med25-VBD-6His

   Plasmid

   #64773

   Purpose

   Bacterial expression of His-tagged Med25-VBD

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25-VBD (MED25 Human)
   

   Expression

   Bacterial

   Mutation

   insert is 394-543 only

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSB63 - pL0_fLUC-I (CDS1)

   Plasmid

   #123180

   Purpose

   Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
   

   Use

   Luciferase; Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-GFP1-10-actin-pcDNA4-TO

   Plasmid

   #118370

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Tags

   HA-GFP1-10

   Expression

   Mammalian

   Promoter

   CMV/TO

   Available Since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCCL-PGK-SPdCas9-BFP-DNMT3B

   Plasmid

   #66820

   Purpose

   dCas9 fused to BFP and the human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
   

   Tags

   dSpCas9-BFP-DNMT3B

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMYC BRGI aa597-1407

    Plasmid

    #19146

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    human BRG1 aa 597-1407 (PCR product)

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTBL469

    Plasmid

    #126458

    Purpose

    Expresses Cas9-15xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    15x Flag
    TdT (DNTT Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDN-D2irG4kwh

    Plasmid

    #44723

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    EGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid

    #126473

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMYC BRGI aa1400-1648

    Plasmid

    #19145

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    human BRG1 aa 1400-1648 (PCR product)

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

    Plasmid

    #71219

    Purpose

    dCas9 fused to the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIE-C-hα2M-H6

    Plasmid

    #128494

    Purpose

    Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (R787-N1473) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    contains R787-N1473

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUPER-retro-puro-Smad2

    Plasmid

    #89827

    Purpose

    Knockdown human Smad2 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    SMAD2 shRNA (SMAD2 Human)
    

    Use

    Retroviral

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. His6-SMT3-SopB(C460S)

    Plasmid

    #183677

    Purpose

    Recombinant Salmonella Typhimurium SopB (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    C460S

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS L30 Ruby3-Rab7A

    Plasmid

    #135651

    Purpose

    Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    Ruby3

    Expression

    Mammalian

    Promoter

    L30

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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