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pcDNA3-myc-Skp2

Plasmid

#19947

Depositor

Yue Xiong

Article

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Tags

myc

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pmEGFP-DRD2

Plasmid

#190752

Purpose

Expression of DRD2 with mEGFP tag on N-terminus.

Depositor

Dave Piston

Article

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Insert

DRD2 (DRD2 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-GFP-HiBiT

Plasmid

#198317

Purpose

Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-GFP-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm4b-pA

Plasmid

#104486

Purpose

Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

Depositor

Yi Zhang

Article

Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

Insert

Kdm4b (Kdm4b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSL4393 (pClpX_Eco)

Plasmid

#200907

Purpose

Expresses human codon-optimized E. coli ClpX from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

EcoClpX

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-truncated

Plasmid

#198286

Purpose

Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-truncated

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSL3713 (pTnsC_Pse)

Plasmid

#200900

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsC

Tags

3xNLS

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-Tubulin.K40Q

Plasmid

#32912

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

Tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40Q

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pROSA26-CreERT2

Plasmid

#149437

Purpose

ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-3XMyc-mTrim25

Plasmid

#51394

Depositor

Narry Kim

Article

Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

Insert

Trim25 (Trim25 Mouse)

Use

Lentiviral

Tags

3XMyc

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pHR hEF1A-BFP-P2A-rtTA-WPRE

Plasmid

#223288

Purpose

A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:

Insert

BFP-P2A-rtTA

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-epNTR

Plasmid

#62213

Purpose

epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

Depositor

Harold Burgess

Article

Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

Insert

epNTR

Use

Mrna synthesis

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre-HiBiT

Plasmid

#198318

Purpose

Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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G555

Plasmid

#216483

Purpose

Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

Depositor

Christophe Danelon

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

plsB, plsC, cdsA, pssA

Use

Cell-free expression

Promoter

T7 for each gene

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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hNCAM2

Plasmid

#84473

Purpose

expression of hNCAM2 in mammalian cells

Depositor

Vladimir Sytnyk

Article

Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

Insert

NCAM2 (NCAM2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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NPC1-CFP P692S

Plasmid

#58457

Purpose

expression of NPC1-CFP sterol sensing domain mutant

Depositor

Matthew Scott

Article

Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

Insert

NPC1 (Npc1 Mouse)

Tags

ECFP and His6

Expression

Mammalian

Mutation

P692S sterol sensing domain mutant

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

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pmPB3-VSV

Plasmid

#68039

Purpose

Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Tags

VSV

Expression

Mammalian

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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