We narrowed to 7,570 results for: ALP

1. pGG-GTS-TurboID

   Plasmid

   #209397

   Purpose

   Transiently expressing TurboID fused with a Golgi targeting sequence (GTS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   GTS-3XHA-TurboID
   

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available Since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MT2A_beta-domain

   Plasmid

   #200300

   Purpose

   Bacterial expression plasmids for production of recombinant beta-domain of metallothionein 2a

   Depositor

   Artur Krężel

   Article

   Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

   Insert

   beta-domain of metallothionein 2a (MT2A Human)
   

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-TdIF2

   Plasmid

   #196906

   Purpose

   visualization of TdIF2/ERBP as a fusion to mCherry

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   TdIF2
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-TdIF2

   Plasmid

   #199699

   Purpose

   visualization of nucleolus in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   TdIF2
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTubulin-miRFP718nano

   Plasmid

   #195746

   Purpose

   Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   miRFP718nano-human alpha-tubulin
   

   Tags

   miRFP718nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. OA-1085L

   Plasmid

   #191376

   Purpose

   pBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomato

   Depositor

   Omar Akbari

   Article

   Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

   Insert

   4 gRNAs targeting GFP
   

   Expression

   Insect

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. OA-1163A

   Plasmid

   #194001

   Purpose

   pBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed.

   Depositor

   Omar Akbari

   Article

   Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

   Insert

   Uniq-CasRx (Codon optimized)
   

   Expression

   Insect

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. OA-1050T

   Plasmid

   #191374

   Purpose

   pBac-AedesUbiq-RfxCas13d-p10-Opie2-dsRed.

   Depositor

   Omar Akbari

   Article

   Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

   Insert

   Uniq-CasRx
   

   Expression

   Insect

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

   Plasmid

   #186708

   Purpose

   N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

   Depositor

   Paula Tamagnini

   Article

   Mascia et al Green Chemistry, 24 (2022):6156

   Insert

   alr4766
   

   Use

   Synthetic Biology

   Tags

   His-Tag (6His)

   Promoter

   PpsbA2*

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

    Plasmid

    #186710

    Purpose

    N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    His-Tag (6His)

    Promoter

    Ptrc.x.tetO2

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid

    #181976

    Purpose

    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

    Depositor

    Jan-Henning Klusmann

    Inserts

    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP_Lims (MBP_LIM1234)

    Plasmid

    #182042

    Purpose

    For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1234 (PXN Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    LIM domains 1-4 only

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST_LIM234

    Plasmid

    #182039

    Purpose

    For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM234 (PXN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LIM domains 2-4 only

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBlastR-GMRWhite

    Plasmid

    #165885

    Purpose

    Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPuroR-GMRWhite

    Plasmid

    #165884

    Purpose

    Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrichoGate-3

    Plasmid

    #160703

    Purpose

    tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    tef1-alpha
    

    Use

    Synthetic Biology

    Mutation

    Incorporation of BsaI restriction sites

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

    Plasmid

    #139964

    Purpose

    Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202
    

    Use

    Synthetic Biology

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-LAP1-mCherry

    Plasmid

    #159525

    Purpose

    Expresses mCherry from LAP1 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    LAP1

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-Myosin7B-sgRNA

    Plasmid

    #154861

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    MYO7B sgRNA (MYO7B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBz172_CsoS2_N1_GFP

    Plasmid

    #140852

    Purpose

    His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 1 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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