We narrowed to 4,881 results for: phy

1. pTL-Bag4-gRNA4

   Plasmid

   #196255

   Purpose

   Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTL-Bag2T-gRNA2

   Plasmid

   #196253

   Purpose

   Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPKm-118

   Plasmid

   #90491

   Purpose

   Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   pFR Luciferase Fluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-C1X-Nup93-mut

   Plasmid

   #87335

   Purpose

   To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP93 (NUP93 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLJM1-Rap2A-3xFLAG

   Plasmid

   #87973

   Purpose

   Expression of Rap2A in mammalian cells

   Depositor

   David Sabatini

   Article

   Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY71-tet.I.8

   Plasmid

   #133521

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L2-AA012

   Plasmid

   #185751

   Purpose

   Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

   Depositor

   Peter Szoevenyi

   Article

   Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

   Inserts

   HygR2
   eGFP
   

   Use

   Tags

   membrane localization tag Lti6b

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MAC_C_EPHA8

   Plasmid

   #187761

   Purpose

   MAC-tagged gene expression of human EPHA8

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   EPHA8 (EPHA8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEos2-CD151-7

   Plasmid

   #57358

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   CD151 (CD151 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pOpLexA::mini35s (GB0733)

    Plasmid

    #68180

    Purpose

    Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    4xOpLexA:mini35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDGB1omega2_SF (GB0466)

    Plasmid

    #68243

    Purpose

    Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB1alpha2_SF (GB0107)

    Plasmid

    #68233

    Purpose

    Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTHsp18.2 (GB0035)

    Plasmid

    #68186

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3xFLAG-CMV-zDicer

    Plasmid

    #171539

    Purpose

    Encodes Zebrafish Dicer protein

    Depositor

    Yan Zeng

    Article

    Li et al Biochem Biophys Res Commun. 2019 Jun 18;

    Insert

    dicer1(MK642699) (dicer1 Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mEos2-FilaminA-C-14

    Plasmid

    #57378

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    FilaminA (FLNA Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    D2626H

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pOpLacI:mini35ss (GB0534)

    Plasmid

    #68179

    Purpose

    Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    6xOpLacI:mini35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUB6_EPB49

    Plasmid

    #84015

    Purpose

    EPB49 minigene to test splicing event

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    EPB49 (DMTN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UB6

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFE-vcDAB

    Plasmid

    #133004

    Purpose

    pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    vcdabB
    vcdabA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-HA-human MYCN-V421D

    Plasmid

    #74166

    Purpose

    Mammalian expression of human MycN-VD mutant

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V421D

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pET29a-hNABP2-N

    Plasmid

    #128308

    Purpose

    Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    hNABP2 N terminal fragment (OB fold) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal domain (1–138 aa)

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge