We narrowed to 4,941 results for: puc

1. qTAG-C-3xHA-Zeo

   Plasmid

   #207740

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. qTAG-N-Zeo-3xHA

   Plasmid

   #207707

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TC1405

   Plasmid

   #164877

   Purpose

   BFP-preSTOP-GFP reporter

   Depositor

   Tian Chi

   Article

   Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

   Insert

   BFP-preSTOP-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pADH110

   Plasmid

   #90982

   Purpose

   NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   NAT 2 of 2
   pSNR52
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAPT_Halo_C_allele

   Plasmid

   #178143

   Purpose

   Donor vector for endogenous tagging of human MAPT at the C-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Halotag (MAPT Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCalpha EV (PKA catalytic subunit Calpha)

   Plasmid

   #15310

   Depositor

   Stanley McKnight

   Article

   Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

   Insert

   PRKACA (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pEF1α-Cre-2A-miRFP670nano3

   Plasmid

   #207747

   Purpose

   Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-PGK-miRFP670nano3
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTX1-RNA0

   Plasmid

   #126040

   Purpose

   In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   RNA0
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGCG26

   Plasmid

   #82371

   Purpose

   YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTn7xTS-sfGFP

    Plasmid

    #117390

    Purpose

    Tn7 tagging vector pTn7xTS with sfGFP expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-moxGFP-Blast

    Plasmid

    #207710

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Enhanced Repressor Domain for TAL Effector (SID4X)

    Plasmid

    #43882

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    SID4X Domain
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

13. UAS:iGluSnfr.zf1

    Plasmid

    #108356

    Purpose

    UAS driven variant of iGluSnFR for zebrafish expression

    Depositor

    Harold Burgess

    Article

    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

    Insert

    UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-N-Zeo-ultraID

    Plasmid

    #207704

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. S5_EcoRV_Ef1a_AscI

    Plasmid

    #128469

    Purpose

    Ef1a promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    Ef1a promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLS485

    Plasmid

    #188890

    Purpose

    Worm Cas9 expression vector, Peft-3 promoter

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Peft-3::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH220

    Plasmid

    #122836

    Purpose

    dCas9 with GreenGate B-C flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Contains D10A and H840A mutations

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTn7-SCOUT13.G

    Plasmid

    #201001

    Purpose

    pLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. LI Intron c1

    Plasmid

    #54433

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    Intron element for construction of silencing vectors (intron1 of AtWRKY33)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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