We narrowed to 12,111 results for: LEA

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pVoPo-02

Plasmid

#187837

Purpose

Backbone for translational reporter system in Fusobacterium nucleatum

Depositor

Jörg Vogel

Publication

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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HA-BACE1-mBFP

Plasmid

#196697

Purpose

Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Publication

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Beta-secretase 1 (BACE1 Human)

Tags

HA and mBFP

Expression

Mammalian

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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766 p3E-2A-nlsmCherrypA

Plasmid

#195972

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-nlsmCherry (nuclear)

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

Plasmid

#169187

Purpose

To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Publication

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Human 3' HP1a AID GFP PuroR

Plasmid

#127906

Purpose

HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

Depositor

Mark Groudine

Publication

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

HP1 (CBX5 Mustard Weed, Human)

Use

Donor plasmid

Tags

GFP, AID, PuroR

Mutation

Inserting GFP AID 2A Puro into mouse 3' Hp1a

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCEP4-HA-CCR5

Plasmid

#98950

Purpose

Mammalian expression plasmid for N-terminal HA-tagged human CCR5

Depositor

Erik Procko

Publication

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

HA (YPYDVPDYA) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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764 pME nls-mApple

Plasmid

#195962

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple nuclear

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Membrane EGFP

Plasmid

#198056

Purpose

Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence

Depositor

Amro Hamdoun

Insert

LCK membrane EGFP (LCK A. victoria, Synthetic, Human)

Use

Sea urchin, mammal, zebrafish

Tags

EGFP

Mutation

Tandem repeat of N-terminal LCK membrane targetin…

Promoter

sp6

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Rac1

Plasmid

#83359

Purpose

Expression of Antenna-Rac1

Depositor

Klaus Hahn

Publication

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Rac1 (RAC1 Human)

Tags

FRET Antenna (mCerulean-cpVenus) and His

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSc1-DD

Plasmid

#80439

Purpose

Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Publication

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Tags

E. coli dihydrofolate reductase

Expression

Mammalian

Promoter

CMV and U6

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICSL11055

Plasmid

#68252

Purpose

Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos

Use

Synthetic Biology

Expression

Plant

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBABE-Flag-Cdk9-T186A-IRES-eGFP

Plasmid

#28097

Depositor

Andrew Rice

Publication

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Contains T186A mutation in Cdk9

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBABE-Flag-Cdk9-D167N-IRES-eGFP

Plasmid

#28098

Depositor

Andrew Rice

Publication

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Contains D167N mutation in Cdk9

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMSCV PIG IMP-1-long-mut

Plasmid

#21661

Depositor

David Bartel

Publication

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Mutation

mutated let-7 binding sites.

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Hyg)

Plasmid

#110077

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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RP1B

Plasmid

#26563

Depositor

Wolfgang Peti

Publication

Peti et al Protein Expr Purif. 2007 Jan . 51(1):1-1

Type

Empty backbone

Tags

His6, TEV cleavage site, and Thio-6

Expression

Bacterial

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pGBW-m4046921

Plasmid

#145755

Purpose

Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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