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Showing: 3041 - 3060 of 3073 results

  5. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  8. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236230-plasmid-map-sequence-id-471247

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  10. Myc-Bnip3-T181A

    Plasmid
    #197556
    Purpose
    Mammalian expression of myc-tagged mouse Bnip3 with an alanine substitution of threonine-181. Based on Myc-Bnip3FL (#100796)
    Depositor
    Joseph Gordon
    Article
    Martens et al Cell Death Dis. 2021 Nov 26;12(12):1105.
    Insert
    BCL2 Interacting Protein 3 (Bnip3 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Threonine 181 changed to alanine
    Promoter
    CMV
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197556-plasmid-map-sequence-id-386257

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Showing: 3041 - 3060 of 3073 results