We narrowed to 14,461 results for: EGF

1. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-en31FnCas9ABEmax8.17d

   Plasmid

   #201956

   Purpose

   Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-3xHA-dFnCas9

   Plasmid

   #201954

   Purpose

   Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dFnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A and H969A on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-3xHA-dSpCas9

   Plasmid

   #201953

   Purpose

   Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dSpCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A on SpCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-3xHA-SpCas9-HF1

   Plasmid

   #201952

   Purpose

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458-3xHA-en1FnCas9

   Plasmid

   #201947

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en1FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1369R on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458-3xHA-FnCas9ABEmax8.17d

   Plasmid

   #201955

   Purpose

   Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. G protein alpha-s-GFP

    Plasmid

    #66083

    Purpose

    G protein alpha-s internally tagged with EGFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-EGFP (Gnas Rat)
    

    Tags

    EGFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1580

    Plasmid

    #29266

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple109 (HBEGF Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pUMN105

    Plasmid

    #233024

    Purpose

    Encodes eGFP for ORBIT integration. High copy number.

    Depositor

    Anthony Baughn

    Type

    Empty backbone

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-Cre

    Plasmid

    #24064

    Purpose

    Retroviral coexpression of Cre and EGFP

    Depositor

    Dan Littman

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    MESV 5' LTR

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNK-TGCK

    Plasmid

    #45359

    Depositor

    Sean Wu

    Inserts

    tTA
    eGFP-Cre
    

    Expression

    Mammalian

    Promoter

    Mouse Nkx2.5 cardiac enhancer and promoter fragme…

    Available Since

    Aug. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Kv2.2

    Plasmid

    #231678

    Purpose

    Expression of mouse Kcnb2 fused with EGFP in C-terminal.

    Depositor

    Federico Sesti

    Insert

    Kcnb2 (Kcnb2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    None

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2B-58

    Plasmid

    #177928

    Purpose

    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    EGFP
    

    Expression

    Bacterial and Yeast

    Promoter

    TET4 (contains four rtTA binding sites)

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2B-57

    Plasmid

    #177927

    Purpose

    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    EGFP
    

    Expression

    Bacterial and Yeast

    Promoter

    TET3 (contains three rtTA binding sites)

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLP-mOlig2-ires-gfp

    Plasmid

    #49399

    Purpose

    mammalian bicistronic expression of mouse Olig2 and EGFP

    Depositor

    Randall McKinnon

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP and IRES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lck-GFP

    Plasmid

    #61099

    Purpose

    Expression of a membrane-targeted form of eGFP

    Depositor

    Steven Green

    Article

    Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

    Insert

    Lck-GFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABEmax-SpRY

    Plasmid

    #195279

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

    Depositor

    Carsten Lederer, Petros Patsali

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-T7-ABEmax-SpG

    Plasmid

    #195278

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

    Depositor

    Carsten Lederer, Petros Patsali

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGFPCPB1

    Plasmid

    #23009

    Depositor

    Linda Wordeman

    Article

    Wordeman et al J Cell Biol. 2007 Dec 3. 179(5):869-79.

    Insert

    EGFP-CenpB
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted CenpB amino acids 169-606, T145S

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRB30

    Plasmid

    #72860

    Purpose

    template for N-terminal eGFP tag, inducible Blasticidin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    eGFP
    

    Expression

    Insect

    Mutation

    eGFP

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLT1C-c201

    Plasmid

    #205108

    Purpose

    FLT1 protein expression

    Depositor

    Paul Brennan

    Insert

    FLT1 (FLT1 Human)
    

    Tags

    AviTag-6His and PTPRSsp

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ATF4 5: 5’ATF4.GFP

    Plasmid

    #21852

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Aiolos ZF2 (AA146-168) in Cilantro 2

    Plasmid

    #74456

    Purpose

    Monitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Aiolos AA130-189 in Pea

    Plasmid

    #74459

    Purpose

    Monitor post-translational degradation of Aiolos AA130-189 via EGFP:mCherry ratio (Lentiviral SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJW1515

    Plasmid

    #62385

    Purpose

    pFA6a Osm1-yoEGFP::LEU2

    Depositor

    Jonathan Weissman

    Article

    Williams et al Science. 2014 Nov 7;346(6210):748-51. do

    Insert

    OSM1-EGFP fusion
    

    Tags

    EGFP

    Expression

    Yeast

    Promoter

    Adh1p

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pITGA4-GFP

    Plasmid

    #81178

    Purpose

    Mammalian expression of human full length ITGA4-EGFP

    Depositor

    Chinten James Lim

    Insert

    ITGA4 (ITGA4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains KLGFFKR motif; A1002L (please see deposi…

    Promoter

    CMV

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJA291

    Plasmid

    #74487

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    puroR-mCherry-T2A-eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJW1517

    Plasmid

    #62387

    Purpose

    pFA6a Osm1(M33A)-yoEGFP::LEU2

    Depositor

    Jonathan Weissman

    Article

    Williams et al Science. 2014 Nov 7;346(6210):748-51. do

    Insert

    OSM1-EGFP fusion
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Methionine 33 mutated to Alanine

    Promoter

    Adh1p

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJW1516

    Plasmid

    #62386

    Purpose

    pFA6a Osm1(M1A)-yoEGFP::LEU2

    Depositor

    Jonathan Weissman

    Article

    Williams et al Science. 2014 Nov 7;346(6210):748-51. do

    Insert

    OSM1-EGFP fusion
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Methionine 1 to Alanine

    Promoter

    Adh1p

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKM493

    Plasmid

    #140191

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    TEV-Flag-Gly4-eGFP
    

    Tags

    Flag + eGFP

    Expression

    Bacterial

    Promoter

    PGroEL

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 7TG

    Plasmid

    #24314

    Depositor

    Roel Nusse

    Article

    Brugmann et al Development. 2007 Sep . 134(18):3283-95.

    Insert

    7xTcf-eGFP
    

    Use

    Lentiviral

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBEL2108

    Plasmid

    #201969

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

    Insert

    attB-3C-eGFP-4xGly-TEV-Flag-6xHis
    

    Use

    Orbit integrating plasmid

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNSEN-d2

    Plasmid

    #59763

    Purpose

    Expresses nuclear EGFP and Tomato in mammalian cells

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

    Inserts

    d2-EGFP
    Tomato
    

    Tags

    3X NLS and PEST

    Expression

    Mammalian

    Promoter

    CMV promoter and SV40 promoter

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDT305

    Plasmid

    #128801

    Purpose

    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    

    Use

    CRISPR and Lentiviral

    Promoter

    hUBC promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEMS-II

    Plasmid

    #204765

    Purpose

    GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    EGFP-m6Alinker-DHFR
    

    Mutation

    mutated all RACs in EGFP to RGC/RCC

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GAG-Pan

    Plasmid

    #99640

    Purpose

    EGFP-mCherry Met71GAG reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Met71 mutated to GAG (Glu)

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GAA-Pan

    Plasmid

    #99639

    Purpose

    EGFP-mCherry Met71GAA reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Met71 mutated to GAA (Glu)

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMaCTag-05

    Plasmid

    #119984

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJAT39

    Plasmid

    #204289

    Purpose

    For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-EGFP+H3:H26
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. WT-Pan

    Plasmid

    #99638

    Purpose

    EGFP-mCherry control reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLPS-hSox2-gfp

    Plasmid

    #49390

    Purpose

    mammalian expression of human Sox2 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    CMV

    Available Since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJAT75

    Plasmid

    #204306

    Purpose

    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCellFree_G03

    Plasmid

    #67137

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis eGFP tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and His

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid

    #192838

    Purpose

    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Insert

    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pJAT43

    Plasmid

    #204299

    Purpose

    For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCMB-ACTLg

    Plasmid

    #61195

    Purpose

    Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-eGFP
    

    Tags

    eGFP

    Expression

    Plant

    Promoter

    MtBCP1

    Available Since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCellFree_G04

    Plasmid

    #67138

    Purpose

    Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and HIS

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge