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We narrowed to 9,672 results for: Gnas

Showing: 7701 - 7750 of 9672 results

  2. mbYFPQS

    Plasmid
    #80742
    Purpose
    membrane-targeted biosensor for monitoring changes in intracellular chloride
    Depositor
    Susan Amara, Susan Ingram
    Article
    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371
    Insert
    YFPQS
    Use
    Tags
    palmitoylation signal sequence of neuromodulin
    Expression
    Mammalian
    Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
    Promoter
    CMV
    Available since
    Aug. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pMpGWB115

    Plasmid
    #68569
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Nuclear localization signal-tagged Citrine (Citri…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68569-plasmid-map-sequence-id-121797

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  6. ULK1-Halo HRD

    Plasmid
    #207548
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    207548-plasmid-map-sequence-id-430489

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  11. pMpGWB116

    Plasmid
    #68570
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Nuclear localization signal-tagged tdTomato (tdTo…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68570-plasmid-map-sequence-id-128992

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  12. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  14. pTEV5_ RpMatB_2

    Plasmid
    #78206
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A S170A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A S170A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78206-plasmid-map-sequence-id-146045

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  16. FRB_LD 0_CS G_tTA-BFP

    Plasmid
    #58876
    Purpose
    MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58876-plasmid-map-sequence-id-99612

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  19. pCX-SpiCee-Kras

    Plasmid
    #140838
    Purpose
    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Article
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Insert
    SpiCee-Kras
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140838-plasmid-map-sequence-id-304816

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  23. pEGFP_D2A_tdTomato

    Plasmid
    #184045
    Purpose
    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)
    Depositor
    Angela Pannier
    Article
    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10
    Inserts
    Enhanced Green Fluorescent Protein
    tdTomato
    Dual 2A Peptide Sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    184045-plasmid-map-sequence-id-363159

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  24. pMpGWB215

    Plasmid
    #68606
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Nuclear localization signal-tagged Citrine (Citri…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68606-plasmid-map-sequence-id-121798

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  27. IMS-HyPer2

    Plasmid
    #60248
    Purpose
    Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.
    Depositor
    Vsevolod Belousov
    Article
    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.
    Insert
    IMS-HyPer2
    Use
    Tags
    Mitochondrial intermembrane space targeting signa…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    60248-plasmid-map-sequence-id-317297

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  30. pDS275

    Plasmid
    #34972
    Purpose
    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).
    Depositor
    Michael Glotzer
    Article
    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm
    Insert
    Mid2(SS/TM)-GFP-LOVpep
    Use
    Tags
    Expression
    Yeast
    Mutation
    T406A, T407A, I532A
    Promoter
    Available since
    March 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    34972-plasmid-map-sequence-id-42929

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  41. pET22b_EcSSB_1

    Plasmid
    #78203
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1
    Insert
    ssb (G26C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G26C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78203-plasmid-map-sequence-id-151729

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  47. pET28a-B4E

    Plasmid
    #162067
    Purpose
    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E
    Depositor
    Karen Polizzi
    Article
    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1
    Insert
    ampR-eIF4E fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    eIF4E incorporating the K119A mutation, which is …
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162067-plasmid-map-sequence-id-325536

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  48. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid
    #216309
    Purpose
    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216309-plasmid-map-sequence-id-433372

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Showing: 7701 - 7750 of 9672 results