We narrowed to 13,968 results for: SHI

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pAB035a

Plasmid

#79190

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-SH2ABL1

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

Dec. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HA-Amida

Plasmid

#19794

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

Amida (TFPT Human)

Tags

2XHA

Expression

Mammalian

Available Since

Nov. 24, 2008

Availability

Academic Institutions and Nonprofits only
BacPAK Flag-NFRKB

Plasmid

#19791

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

Flag and His

Expression

Insect

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Hy_pMT eGFP-RVFV NSs HhRz

Plasmid

#91801

Purpose

Expresses a non-polyadenylated, translatable EGFP mRNA in Drosophila

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

EGFP

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-Lysosomes-20

Plasmid

#55495

Purpose

Localization: Lysosome Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LAMP1

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6_rpoA-C

Plasmid

#190986

Purpose

Test the C-to-U editing activity of KP6 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP2_rpoA-U

Plasmid

#190976

Purpose

Test the U-to-C editing activity of KP2 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP3_rpoA-U

Plasmid

#190980

Purpose

Test the U-to-C editing activity of KP3 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2_rpoA-U

Plasmid

#190956

Purpose

Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-A66S_rpoA-U

Plasmid

#190969

Purpose

Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3_rpoA-U

Plasmid

#190960

Purpose

Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-E67A_rpoA-U

Plasmid

#190973

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(2,100,50)-GFP

Plasmid

#124622

Purpose

Expresses adRNA(2,100,50) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(2,100,50)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(0,100,50)-GFP

Plasmid

#124623

Purpose

Expresses adRNA(0,100,50) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(0,100,50)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-FAK-5

Plasmid

#55484

Purpose

Localization: Focal Adhesion Kinase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

FAK

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-LC-Myosin-N-7

Plasmid

#55494

Purpose

Localization: Light Chain Myosin, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LC-Myosin

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mTFP1-Paxillin-22

Plasmid

#55504

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Paxillin

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
BII-ChBtW-PLAGL2

Plasmid

#133369

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA

Plasmid

#124624

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MS2_adRNA_RAB7A

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmXRN1_A

Plasmid

#145874

Purpose

Insect Expression of DmXRN1

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmXRN1 (pcm Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET-GST-05230

Plasmid

#67874

Purpose

E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.

Depositor

Armin Djamei

Article

Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

Insert

shy1

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PI(LM)-Rac1(WT)dc1.FLARE

Plasmid

#87590

Purpose

PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pX459V2.0+K848A

Plasmid

#108296

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3927-64

Plasmid

#176500

Purpose

pTRE-EGFP-T2A-TFEB(delta30; amino acids 31-476)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(human; delta30; amino acids 31-476)

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29NK-LGK/mGFPmut3

Plasmid

#81168

Purpose

GFP fusion to levoglucosan kinase

Depositor

Timothy Whitehead

Article

Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

Insert

Levoglucosan kinase wild-type

Tags

His6x and mGFPmut3

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECB

Plasmid

#106305

Purpose

Express spCas9 with sgRNA, blast resistance

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript-mHMGB4

Plasmid

#31620

Depositor

Yasuhiko Kawakami, Martin Lotz

Article

Itou et al Dev Dyn. 2011 May . 240(5):1151-62.

Insert

HMGB4 (Hmgb4 Mouse)

Expression

Bacterial

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pAB029f

Plasmid

#79220

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-SH2ABL1; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB031a

Plasmid

#79221

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-Zif268; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2

Plasmid

#79255

Purpose

Expresses EGFP-tagged DmNot2 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot2 (Rga Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot3

Plasmid

#79250

Purpose

Expresses EGFP-tagged DmNot3 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot3 (Not3 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Human SOX17 sgRNA

Plasmid

#59725

Purpose

Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hSox17 sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pDM3193

Plasmid

#113390

Purpose

Alpha factor inducible Ty3 with his3AI

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3-his3AI

Tags

his3AI

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mTFP1-Cx26-7

Plasmid

#55473

Purpose

Localization: Gap Junctions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Cx26

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-GFP (F64L, S65T, V163A)-kanMX6

Plasmid

#53183

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

kanMX6

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Profilin-10

Plasmid

#55506

Purpose

Localization: Cytoskeleton, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Profilin

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Human BLIMP1 sgRNA

Plasmid

#59724

Purpose

Plasmid encoding sgRNA to generate BLIMP1 knockout mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hBLIMP1 sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pYPQ293 (BhCas12b_v4)

Plasmid

#136383

Purpose

Gateway entry clone for BhCas12b_v4

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BhCas12b_v4

Use

CRISPR

Expression

Plant

Mutation

K846R/S893R/E837G

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GluR2_adRNA_(1,20,6)-GFP

Plasmid

#124619

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(1,20,6)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CALCOCO1 V142A-mycDDK

Plasmid

#137768

Purpose

CALCOCO1 CLIR mutant

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 V142A

Tags

mycDDK

Expression

Mammalian

Mutation

V142A

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-ADA add back mutant pCMV5

Plasmid

#17554

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

FLAG-FoxO1-ADA

Tags

FLAG

Expression

Mammalian

Mutation

siRNA-resistant Foxo1 by replacing 3 residues (un…

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only
mTFP1-VASP-5

Plasmid

#55513

Purpose

Localization: Actin/Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VASP

Tags

mTFP1

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pAB043c

Plasmid

#79223

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-p38-alpha; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 13,968 results for: SHI