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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 17,767 results for: REV

Showing: 3081 - 3100 of 17767 results

  1. D282N Cus2p

    Plasmid
    #73060
    Purpose
    S. cerevisiae Cus2p gene with D282N Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Asp 282 to Asn
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    73060-plasmid-map-sequence-id-150798

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  3. LV-PE_CO-Mini

    Plasmid
    #190338
    Purpose
    Lentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase)
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini, ngRNA and pegRNA for HEK3 CTTins
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    190338-plasmid-map-sequence-id-374563

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  5. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  9. PhyB-mCherry-Tom7

    Plasmid
    #66571
    Purpose
    Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae
    Depositor
    Orion Weiner
    Article
    Jost et al ACS Synth Biol. 2015 Jun 2.
    Insert
    PhyB (PHYB Mustard Weed)
    Use
    Tags
    Tom7 and mCherry
    Expression
    Yeast
    Mutation
    PhyB portion contains aa's 1-908
    Promoter
    pADH1
    Available since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66571-plasmid-map-sequence-id-126395

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  18. cPE

    Plasmid
    #183549
    Purpose
    Expresses compact Prime Editor in mammalian cells
    Depositor
    Wen Xue
    Article
    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do
    Insert
    SpCas9 nickase (H840A), Reverse Transcriptase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183549-plasmid-map-sequence-id-365389

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  19. Let7R-SunTag

    Plasmid
    #196929
    Purpose
    Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.
    Depositor
    Jeffrey Chao
    Article
    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.
    Insert
    Renilla luciferase
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196929-plasmid-map-sequence-id-387449

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Showing: 3081 - 3100 of 17767 results