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MSGV Hu Acceptor

Plasmid

#64269

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

Depositor

David Ott

Publication

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

none

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAV-mRuby2-Gephyrin.FingR-IL2RGTC

Plasmid

#125696

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Publication

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mRuby2-Gephyrin.FingR-IL2RGTC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Publication

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Gateway Cloning

Expression

Mammalian

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCRT

Plasmid

#120274

Purpose

TA-cloning vector of PCR-products

Depositor

Ken Motohashi

Publication

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCRZero

Plasmid

#120275

Purpose

Blunt-end cloning vector of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Publication

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV PIG IMP-1-short

Plasmid

#21659

Depositor

David Bartel

Publication

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV5 MEKK2 WT

Plasmid

#12182

Depositor

Gary Johnson

Publication

Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MEKK2 (Map3k2 Mouse)

Tags

HA

Expression

Mammalian

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEG BacMam N term His8 eGFP 3C

Plasmid

#160684

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB414

Plasmid

#74808

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Luc-noPS

Plasmid

#177940

Purpose

Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Luc2

Expression

Mammalian

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC

Plasmid

#125695

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Publication

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mScarlet-Gephyrin.FingR-IL2RGTC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Publication

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDuRCC-K

Plasmid

#81193

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Publication

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below., Please view…

Promoter

ROX3 and SNP52p

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 V5 hHR23A

Plasmid

#13053

Depositor

Steven Grossman

Publication

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hHR23A (RAD23A Human)

Tags

His and V5

Expression

Mammalian

Mutation

R325K and L355P relative to NP_001257291.1

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLEX307-FURIN-puro

Plasmid

#158460

Purpose

Constitutive lentiviral expression of human FURIN.

Depositor

Alejandro Chavez, Sho Iketani

Insert

FURIN (FURIN Human)

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LbABE8e

Plasmid

#138504

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA(8e)-dLbCas12a

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHMGWA-Pa14Csy4H29A

Plasmid

#41092

Depositor

Jennifer Doudna

Publication

Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

Insert

Csy4

Tags

His6-MBP

Mutation

His29Ala mmutation to abolish ribonuclease activi…

Promoter

T7

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMJ839

Plasmid

#39317

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (NMA0631 Neisseria meningitidis)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
UAS-mCherry

Plasmid

#135457

Purpose

Mammalian mCherry reporter to read out FLARE and SPARK activation

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Tags

mCherry

Expression

Mammalian

Promoter

UAS

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Expression

Mammalian

Available since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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