Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 68,975 results for: nin

Showing: 3081 - 3100 of 68975 results

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pCAGEN-FastFUCCI-IRES-myr-mKate2 (JDW 1503)

Plasmid

#242598

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mKate2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+) PCMT1 H58 A214

Plasmid

#246496

Purpose

Recombinant protein expression of PCMT1 H58 A214

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Threonine 58 mutated to Histidine and Valine 214 …

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-mm-miR-500 (JDW 8)

Plasmid

#242563

Purpose

Gateway middle entry clone containing an EGFP fused to the murine microRNA 500.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

GFP with artificial intron and murine microRNA 500 (Mir500 Mouse)

Use

Gateway subcloning

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_PST-C

Plasmid

#228570

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Pancreastatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_PST-N

Plasmid

#228571

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Pancreastatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-C

Plasmid

#228572

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-N

Plasmid

#228573

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_P-linker 4E

Plasmid

#234564

Purpose

CTNNA1 Phospholinker phospho-mimic

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_P-linker 4E (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

S641E, S652E, S655E, T658E

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_P-linker 4A

Plasmid

#234563

Purpose

CTNNA1 Phospholinker phospho-mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_P-linker 4A (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

S641A, S652A, S655A, T658A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_E307K

Plasmid

#234560

Purpose

CTNNA1 butterfly eye mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_E307K (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

E307K

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_R551A

Plasmid

#234558

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_R551A (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

R551A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DNii domain

Plasmid

#234557

Purpose

CTNNA1 Nii-domain deletion mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DNii domain (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Deletion of aa ~165-265

Promoter

CMV

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DM2,3-domain

Plasmid

#234556

Purpose

CTNNA1 M2,3-domain deletion mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DM2,3-domain (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Deletion of aa 397-509

Promoter

CMV

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_DM1-domain

Plasmid

#234555

Purpose

CTNNA1 M1-domain deletion mutant

Depositor

Cara Gottardi

Article

Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

Insert

mEGFP:hCTNNA1_DM1-domain (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Deletion of aa 271-396

Promoter

CMV

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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NST3_pOpOn2.1

Plasmid

#228066

Purpose

Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

Depositor

Charles Anderson

Article

Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

Insert

NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)

Use

Dexamethasone inducible vector

Expression

Plant

Promoter

CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

Plasmid

#224522

Purpose

A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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LTB4R2B-DuET

Plasmid

#213334

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

LTB4R2B (LTB4R2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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