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OPRK1-DuET

Plasmid

#213359

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRK1 (OPRK1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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CYSLTR1-DuET

Plasmid

#213225

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CYSLTR1 (CYSLTR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pC-SUR-YR

Plasmid

#61768

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Sur gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

Magnaporthe oryzae ILV promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCOLADuet-1_nsp10 (SARS-CoV-2)

Plasmid

#169158

Purpose

To express SARS-CoV-2 nsp10 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10 (ORF1ab SARS-CoV-2)

Tags

Methionine

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pUAS-Myc-BirA-G3-ER

Plasmid

#173815

Purpose

Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Depositor

Norbert Perrimon

Article

Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

Insert

Myc-BirA-G3-ER

Tags

Myc

Expression

Insect

Promoter

UAS

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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fck-Jaws-GFP-ER2

Plasmid

#65013

Purpose

Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

Lentiviral

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

Plasmid

#229839

Purpose

A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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HTR1D-DuET

Plasmid

#213318

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HTR1D (HTR1D Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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HTR1B-DuET

Plasmid

#213317

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HTR1B (HTR1B Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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fck-Jaws-KGC-GFP-ER2

Plasmid

#65012

Purpose

Lentivirus-mediated expression of Jaws under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

Lentiviral

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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ADRA2A-DuET

Plasmid

#213177

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADRA2A (ADRA2A Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-dGFP-JARID1C A388P

Plasmid

#74778

Purpose

retroviral expression of JARID1C A388P

Depositor

Giovanni Tonon

Article

Rondinelli et al J Clin Invest. 2015 Dec;125(12):4625-37.

Insert

JARID1C (KDM5C Human)

Use

Retroviral

Expression

Mammalian

Mutation

A388P

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant

Plasmid

#19000

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pTol2-Fli1-zFUCCI-IRES-mTagBFP2 (JDW 1517)

Plasmid

#242602

Purpose

A Tol2 expression vector containing the fli promoter driving FUCCI to label cell cycle in all blood vessels followed by an IRES-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGem-P2A-mCherry-zCdt1, nls-mTagBFP2

Use

Zebrafish expression

Promoter

fli promoter

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mKate2 (JDW 1503)

Plasmid

#242598

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mKate2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+) PCMT1 H58 A214

Plasmid

#246496

Purpose

Recombinant protein expression of PCMT1 H58 A214

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Threonine 58 mutated to Histidine and Valine 214 …

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-mm-miR-500 (JDW 8)

Plasmid

#242563

Purpose

Gateway middle entry clone containing an EGFP fused to the murine microRNA 500.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

GFP with artificial intron and murine microRNA 500 (Mir500 Mouse)

Use

Gateway subcloning

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_PST-C

Plasmid

#228570

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Pancreastatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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