We narrowed to 144,836 results for: ins
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Plasmid#113655PurposesgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.DepositorInsertpromoter T5 and sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterT5Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1 BSMT1
Plasmid#106287PurposeExpresses BSMT1 in Ecoli off a T7 promoterDepositorInsertBSMT1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-144:CTCF-mEGFP
Plasmid#168799PurposeHomology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCFDepositorInsertCTCF (CTCF Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP
Plasmid#67299PurposeBlue light dependent photoswitching systemDepositorInsertTiam1-pMagFast2(3x)-iRFP (Tiam1 Synthetic, Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS AluYa5rescueA70D
Plasmid#78657PurposeGenerates Alu inserts suitable for rescueDepositorInsertYa5rescue cassette
UseTagsExpressionMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-His6-TEV-MMS2
Plasmid#25465DepositorUseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKar2-msGFP-HDEL
Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
UseTagsExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_VP1_RaPID_r1c3
Plasmid#65723PurposeProduce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 onlyDepositorInsertmCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RaACR_687-EYFP
Plasmid#111077PurposeAnion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cellsDepositorInsertAnion channelrhodopsin RaACR_687
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBA323
Plasmid#214751PurposeTo create delta(glmS,nadE) working strains and maintaining their viabilityDepositorInsertBBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-079:DCP1A-mEGFP
Plasmid#193922PurposeHomology arms and mEGFP-SRA for N-terminus tagging of human DCP1ADepositorInsertDCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NpT7-5-Erk2
Plasmid#39230DepositorInsertErk2 (Mapk1 Rat)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCIS-krPAM2
Plasmid#25457DepositorInsertPAM2 (Pam Rat)
UseTagsExpressionMammalianMutationExon A deleted (exon A extends from nt 1475 to n…PromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCOLA kiwi sensor sfGFP
Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEM253 - sgRNA mosTI hygroR
Plasmid#159827PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertsgRNA mosTI hygroR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymVenus-URA3
Plasmid#168058PurposeReplaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymVenus
UseTagsymVenusExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
cacnb1b (rat) in pMT2 vector
Plasmid#107423PurposeExpresses Calcium channel beta1b auxillary subunit in mammalian cells.DepositorInsertcalcium channel beta1b auxillary subunit
UseTagsExpressionMammalianMutationPromoterAd MLPAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDV05.1_pEF1a-iRFP720_frameshifted
Plasmid#200408PurposeExpresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent proteinDepositorInsertiRFP720 (frameshift mutant)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-PhoCl1-CBid
Plasmid#164050PurposeOptogenetic manipulation of cell apoptosis using PhoCl1DepositorInsertNBid-PhoCl1-CBid
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPI452
Plasmid#113203PurposeInducible expression vector (dox-on) N-terminal mScarlet taggingDepositorTypeEmpty backboneUseDictyostelium discoideum inducible expression vec…TagsmScarletExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAGE-A3Pro pGL4basic
Plasmid#78138Purposeluciferase reporter plasmidDepositorInsertMAGE-A3 (MAGEA3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains -418/+12 of promoterPromoterMAGE-A3Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-mPlum
Plasmid#158062PurposeDonor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertmonomeric plum fluoresecent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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NpT7-5-ERK1
Plasmid#39229DepositorInsertERK1 (MAPK3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEM270 - MCS mosTI hygroR target
Plasmid#159826PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertMCS mosTI hygroR target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDM1513
Plasmid#108998Purposesafe haven knock-in plasmid for the act5 locus, N-terminal GFP taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, n…TagsGFPExpressionMutationPromoterAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB5
Plasmid#174624PurposeOptogenetic coupling to early endosomes (RAB5) via iLIDDepositorInsertiLID-mCherry-RAB5 (RAB5A Human, Synthetic)
UseTagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB5: Met1DelPromoterChicken beta-actinAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only