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pScSR beta deltaN Flag

Plasmid

#13729

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

expresses residues 31-244 of S. cerevisiae SRβ wi…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 L306P

Plasmid

#169262

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 L306P (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu306Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 WT

Plasmid

#169265

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 WT (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 L351P

Plasmid

#169266

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 L351P (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu351Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pET41a iGlum

Plasmid

#119829

Purpose

Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlum

Tags

GST

Expression

Bacterial

Mutation

R24K

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pT3-N90-beta-catenin

Plasmid

#31785

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of aa 1-90- Constitutitvely Active

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-TetON-3G

Plasmid

#96963

Purpose

Mammalian expression of Clontech rtTA driven by human beta-actin promoter

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1

Plasmid

#173932

Purpose

Bacterial expression of ALC1

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1 (CHD1L Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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hSOD1-FLAG

Plasmid

#182922

Purpose

Mammalian expression plasmid for human WT SOD1

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

[Cu-Zn] Superoxide dismutase 1 (SOD1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGMCtN-TSC10M1-sspB

Plasmid

#49521

Purpose

Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3 (human)

Plasmid

#24920

Purpose

Mammalian expression of LC3 fused to EGFP

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH c-Met

Plasmid

#86498

Purpose

Mammalian expression of human c-Met

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark3

Plasmid

#233145

Purpose

Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark3

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-Clathrin-15

Plasmid

#54040

Purpose

Localization: Clathrin Vesicles, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Clathrin (CLTB Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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HypoxCR

Plasmid

#59946

Purpose

A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

Depositor

Anne Le

Article

Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

Inserts

GFP-Pest
mCherry-Geminin

Use

Lentiviral

Tags

FLAG and PEST

Expression

Mammalian

Promoter

CMV and min CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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